TAILIEUCHUNG - Báo cáo y học: "Characterization of viroplasm formation during the early stages of rotavirus infection"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Characterization of viroplasm formation during the early stages of rotavirus infection | Carreno-Torres et al. Virology Journal 2010 7 350 http content 7 1 350 VIROLOGY JOURNAL RESEARCH Open Access Characterization of viroplasm formation during the early stages of rotavirus infection José J Carreno-Torres Michelle Gutierrez Carlos F Arias Susana Lopez Pavel Isa Abstract Background During rotavirus replication cycle electron-dense cytoplasmic inclusions named viroplasms are formed and two non-structural proteins NSP2 and NSP5 have been shown to localize in these membrane-free structures. In these inclusions replication ofdsRNA and packaging of pre-virion particles occur. Despite the importance of viroplasms in the replication cycle of rotavirus the information regarding their formation and the possible sites of their nucleation during the early stages of infection is scarce. Here we analyzed the formation of viroplasms after infection of MA104 cells with the rotavirus strain RRV using different multiplicities of infection MOI and different times post-infection. The possibility that viroplasms formation is nucleated by the entering viral particles was investigated using fluorescently labeled purified rotavirus particles. Results The immunofluorescent detection of viroplasms using antibodies specific to NSP2 showed that both the number and size of viroplasms increased during infection and depend on the MOI used. Small-size viroplasms predominated independently of the MOI or time post-infection although at MOI s of and 10 the proportion of larger viroplasms increased. Purified RRV particles were successfully labeled with the Cy5 mono reactive dye without decrease in virus infectivity and the labeled viruses were clearly observed by confocal microscope. PAGE gel analysis showed that most viral proteins were labeled including the intermediate capsid protein VP6. Only 2 out of 117 Cy5-labeled virus particles colocalized with newly formed viroplasms at 4 hours post-infection. Conclusions The results presented in this work suggest that .

TÀI LIỆU LIÊN QUAN
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.