TAILIEUCHUNG - Biosensors Edited byPier Andrea Serra Part 9

Tham khảo tài liệu 'biosensors edited bypier andrea serra part 9', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Bacterial Bioluminescent Biosensor Characterisation for On-line Monitoring of Heavy Metals Pollutions in Waste Water Treatment Plant Effluents 193 Fig. 9. Concentrations in cadmium in membranes without bacteria during several cycles of measure. The bioluminescence curve of bioluminescence with the initial peak is presented only for indication . Experimental conditions Cadmium titrations were realized on membranes without bacteria by ICPMS in the departmental laboratory of analysis of Vendée LDA85 . For each measure the membrane to be analyzed underwent the deliberate number of cycles and was then taken off for analysis. Hence cadmium was not accumulated during all the cycles but a fraction remained blocked in the agarose while this material is not considered as an adsorbant according to Pernetti et al. 2004 . b. Oxygen effect Oxygen is necessary both for the catabolism of the bacteria and the reaction of bioluminescence. The oxygen brought to the bacteria comes only from the assay and the regeneration media and dropped during the biosensor measurement Figure 10 . During the first induction the oxygen concentration at the biosensor output decreased significantly from to whereas it remained stable during the following regeneration . L-1 . The oxygen fall was confirmed with the following cycle and can be at the origin of the decrease of bioluminesence. To address this steady lack of oxygen inside the card a permanent bubbling device was designed to bring air inside the card. Figure 11 compares the results obtained with and without air bubbling. These results show a very significant improvement of the living time of the card with a constant air feeding allowing eleven cycles of measurement instead of five without air bubbling. However the bioluminescence signal disappeared after more than forty operating hours. Oxygen even in excess in the measuring cell did not seem to be the only limiting factor of the living time of the membrane. 194 Biosensors .

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