TAILIEUCHUNG - Rocha et al. AMB Express 2011, 1:9 http://www.amb-express.com/content/1/1/9 ORIGINAL Open

Rocha et al. AMB Express 2011, 1:9 ORIGINAL Open Access Biosurfactant-mediated biodegradation of straight and methyl-branched alkanes by Pseudomonas aeruginosa ATCC 55925 Carlos A Rocha1*, Ana M Pedregosa2 and Fernando Laborda2 Abstract Accidental oil spills and waste disposal are important sources for environmental pollution. We investigated the biodegradation of alkanes by Pseudomonas aeruginosa ATCC 55925 in relation to a rhamnolipid surfactant produced by the same bacterial strain. Results showed that the linear C11-C21 compounds in a heating oil sample degraded from 6% to 100%, whereas the iso-alkanes tended to be recalcitrant unless they were exposed to the biosurfactant; under such condition total biodegradation was. | Rocha et al. AMB Express 2011 1 9 http content 1 1 9 o AMB Express a SpringerOpen Journal ORIGINAL Open Access Biosurfactant-mediated biodegradation of straight and methyl-branched alkanes by Pseudomonas aeruginosa ATCC 55925 Carlos A Rocha1 Ana M Pedregosa2 and Fernando Laborda2 Abstract Accidental oil spills and waste disposal are important sources for environmental pollution. We investigated the biodegradation of alkanes by Pseudomonas aeruginosa ATCC 55925 in relation to a rhamnolipid surfactant produced by the same bacterial strain. Results showed that the linear C11-C21 compounds in a heating oil sample degraded from 6 to 100 whereas the iso-alkanes tended to be recalcitrant unless they were exposed to the biosurfactant under such condition total biodegradation was achieved. Only the biodegradation of the commercial C12-C19 alkanes could be demonstrated ranging from 23 to 100 depending on the experimental conditions. Pristane a C19 branched alkane only biodegraded when present alone with the biosurfactant and when included in an artificial mixture even without the biosurfactant. In all cases the biosurfactant significantly enhanced biodegradation. The electron scanning microscopy showed that cells depicted several adaptations to growth on hydrocarbons such as biopolymeric spheres with embedded cells distributed over different layers on the spherical surfaces and cells linked to each other by extracellular appendages. Electron transmission microscopy revealed transparent inclusions which were associated with hydrocarbon based-culture cells. These patterns of hydrocarbon biodegradation and cell adaptations depended on the substrate bioavailability type and length of hydrocarbon. Keywords Biodegradation patterns alkanes biodegradation biosurfactant P. aeruginosa cell adaptations Introduction Leaking from oil wells tanks pipes and transportation vehicles together with the inadequate waste disposal from the oil industry at large oil exploration

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