TAILIEUCHUNG - Báo cáo y học: "Neuroprotective and anti-oxidant effects of caffeic acid isolated from Erigeron annuus leaf"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Neuroprotective and anti-oxidant effects of caffeic acid isolated from Erigeron annuus leaf. | Jeong et al. Chinese Medicine 2011 6 25 http content 6 1 25 CHINESE MEDICINE RESEARCH Open Access Neuroprotective and anti-oxidant effects of caffeic acid isolated from Erigeron annuus leaf Chang-Ho Jeong1 Hee Rok Jeong2 Gwi Nam Choi2 Dae-Ok Kim1 Uk Lee3 and Ho Jin Heo2 Abstract Background Since oxidative stress has been implicated in a neurodegenerative disease such as Alzheimer s disease AD natural antioxidants are promising candidates of chemopreventive agents. This study examines antioxidant and neuronal cell protective effects of various fractions of the methanolic extract of Erigeron annuus leaf and identifies active compounds of the extract. Methods Antioxidant activities of the fractions from Erigeron annuus leaf were examined with 2 2-azino-bis 3-ethylbenz thiazoline-6-sulfonic acid diammonium salt ABTS and ferric reducing antioxidant power FRAP assays. Neuroprotective effect of caffeic acid under oxidative stress induced by H2O2 was investigated with 3- 4 5-dimethythiazol-2-yl -2 5-diphenyl tetrazolium bromide MTT and lactate dehydrogenase LDH assays. Results This study demonstrated that butanol fraction had the highest antioxidant activity among all solvent fractions from methanolic extract E. annuus leaf. Butanol fraction had the highest total phenolic contents mg of GAE g . Caffeic acid an isolated active compound from butanol fraction showed dose-dependent in vitro antioxidant activity. Moreover neuronal cell protection against oxidative stress induced cytotoxicity was also demonstrated. Conclusion Erigeron annuus leaf extracts containing caffeic acid as an active compound have antioxidative and neuroprotective effects on neuronal cells. Background Oxidative stress refers to the imbalance between the production and removal of reactive oxygen species ROS . Due to the reaction between ROS and macromolecules generation of ROS can lead to damage or death of cells in various tissues 1 . Brain tissue is most vulnerable to oxidative

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