TAILIEUCHUNG - Báo cáo khoa học: An alternative mature form of subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis identified in the presence of Ca2+

Pro-Tk-SP fromThermococcus kodakaraensisconsists of the four domains: N-propeptide, subtilisin (EC ) domain,b-jelly roll domain and C-propeptide. To analyze the maturation process of this protein, the Pro-Tk-SP derivative with the mutation of the active-site serine residue to Cys (Pro-Tk-S359C), Pro-Tk-S359C derivatives lacking the N-propeptide (ProC-Tk-S359C) and both propeptides (Tk-S359C), and a His-tagged form of the isolated C-propeptide (ProC*) were constructed. | IFEBS Journal An alternative mature form of subtilisin homologue Tk-SP from Thermococcus kodakaraensis identified in the presence of Ca2 Nitat Sinsereekul1 Tita Foophow1 Mai Yamanouchi1 Yuichi Koga1 Kazufumi Takano1 2 and Shigenori Kanaya1 1 Department of Materialand Life Science Graduate Schoolof Engineering Osaka University Japan 2 CREST JST Suita Osaka Japan Keywords autoprocessing maturation propeptide subtilisin Thermococcus kodakaraensis Correspondence S. Kanaya Department of Material and Life Science Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita Osaka 565-0871 Japan Fax 81 6 6879 7938 Tel 81 6 6879 7938 E-mail kanaya@ These authors contributed equally to this work Received 24 January 2011 revised 15 March 2011 accepted 23 March 2011 doi Pro-Tk-SP from Thermococcus kodakaraensis consists of the four domains N-propeptide subtilisin EC domain b-jelly roll domain and C-propeptide. To analyze the maturation process of this protein the Pro-Tk-SP derivative with the mutation of the active-site serine residue to Cys Pro-Tk-S359C Pro-Tk-S359C derivatives lacking the N-propeptide ProC-Tk-S359C and both propeptides Tk-S359C and a His-tagged form of the isolated C-propeptide ProC were constructed. Pro-Tk-S359C was purified mostly in an autoprocessed form in which the N-propeptide is autoprocessed but the isolated N-propeptide ProN forms a stable complex with ProC-Tk-S359C indicating that the N-propeptide is autoprocessed first. The subsequent maturation process was analyzed using ProC-Tk-S359C instead of the ProN ProC-Tk-S359C complex. The C-propeptide was autoprocessed and degraded when ProC-Tk-S359C was incubated at 80 C in the absence of Ca2 . However it was not autoprocessed in the presence of Ca2 . Comparison of the susceptibility of ProC to proteolytic degradation in the presence and absence of Ca2 suggests that the C-pro-peptide becomes highly resistant to proteolytic .

• Báo cáo khoa học
• Scientific reports
• scientific research
• Structural biochemistry
• Thermococcus
• bacteria
• medical analysis
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes