TAILIEUCHUNG - Báo cáo y học: "Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI. | Clinical and Molecular Allergy BioMed Central Research Open Access Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FceRI Hans Jurgen Hoffmann 1 Mette Bogebjerg1 2 Lars Peter Nielsen2 and Ronald Dahl1 Address Department of Pulmonary Medicine Aarhus University Hospital Aarhus University DK 8000 Aarhus C Denmark and 2Institute of Pharmacology Aarhus University DK 8000 Aarhus C Denmark Email Hans Jurgen Hoffmann - Mette B0gebjerg - metteboegebjerg@ Lars Peter Nielsen - LPN@ Ronald Dahl - rdahl@ Corresponding author Published 04 July 2005 Received 11 April 2005 Clinical and Molecular Allergy 2005 3 10 doi 1476-7961-3-10 Accepted 04 July 2005 This article is available from http content 3 1 10 2005 Hoffmann et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The basophil activation test BAT in which translocation of markers to the surface of blood basophils is measured in response to allergen by flow cytometry is a rapid assay that is gaining popularity. Two markers are currently being evaluated for the BAT CD63 and the lineagespecific CD203c. In a recent report detection of CD203c after lysis with Saponin was shown to be superior to detection of CD63 after lysis with formic acid. We wanted to compare a lysis with formic acid and lysis with Saponin b the response through CD203c and CD63 and c the definition 10 activated cells above background with the probability binning metric T x 4 on sets of data generated with blood basophils stimulated with varying concentrations of anti-FceRI antibody. .

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