TAILIEUCHUNG - Báo cáo y học: "HIV-1 gene expression: lessons from provirus and non-integrated DNA"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: HIV-1 gene expression: lessons from provirus and non-integrated DNA | Retrovirology BioMed Central Review Open Access HIV-I gene expression lessons from provirus and non-integrated DNA Yuntao Wu Address Center for Biodefense Department of Molecular and Microbiology George Mason University Manassas VA 20110 USA Email Yuntao Wu - ywu8@ Corresponding author Published 25 June 2004 Received 21 May 2004 Retrovirology 2004 1 13 doi 1742-4690-1-13 Accepted 25 June 2004 This article is available from http content 1 1 1 3 2004 Wu licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Replication of HIV-1 involves a series of obligatory steps such as reverse transcription of the viral RNA genome into double-stranded DNA and subsequent integration of the DNA into the human chromatin. Integration is an essential step for HIV-1 replication yet the natural process of HIV-1 infection generates both integrated and high levels of non-integrated DNA. Although proviral DNA is the template for productive viral replication the non-integrated DNA has been suggested to be active for limited viral gene synthesis. In this review the regulation of viral gene expression from proviral DNA will be summarized and issues relating to non-integrated DNA as a template for transcription will be discussed as will the possible function of pre-integration transcription in HIV-1 replication cycle. Introduction Intracellular parasites such as viruses depend on cellular machinery to disseminate their genetic information. Different viruses evolve different strategies to utilize the host machinery. The human immunodeficiency virus HIV prototype of the lentiviral subfamily of Retroviruses is one of the ultimate players in exploiting the host mechanism. Its RNA genome is first reverse transcribed into a DNA template integrated into host chromatin then transcribed

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