TAILIEUCHUNG - The Essential Guide to Image Processing- P28

The Essential Guide to Image Processing- P28:We are in the middle of an exciting period of time in the field of image processing. Indeed, scarcely a week passes where we do not hear an announcement of some new technological breakthrough in the areas of digital computation and telecommunication. | 822 CHAPTER 27 Computer-Assisted Microscopy Deblurring Weinstein and Castleman pioneered the deblurring of optical section images using a simple method that involves subtracting adjacent plane images that have been blurred with an appropriate defocus psf 69 given by M fj gj 52 gj-i h-i gj i hi k0 i 1 where fj is the specimen brightness distribution at focus level j gj is the optical section image obtained at level j hi is the blurring psf due to being out of focus by the amount i k0 is a heuristically designed highpass filter and the represents the convolution operation. Thus one can partially remove the defocused structures by subtracting 2M adjacent plane images that have been blurred with the appropriate defocus psf and convolved with a suitable highpass filter k0. The filter k0 and the number M of adjacent planes must be selected to give good results. While this technique cannot recover the specimen function exactly it does improve optical section images at reasonable computational expense. It is often necessary to use only a small number M of adjacent planes to remove most of the defocused information. Figure shows images from transmitted light microscopy and fluorescence microscopy that have been deblurred using optical sections above and below at a Z-interval of 1 p m. While this technique cannot recover the specimen function exactly it does improve optical section images at reasonable computational expense. FIGURE Deblurring. The top row shows images of FISH-labeled lymphocytes. The left three images are from an optical section stack taken one micron apart. The right image is the middle one deblurred. The in-plane dots are brighter while the out-of-plane dots are removed. The bottom row shows transmitted images of May-Giemsa stained blood cells. The left three images are from an optical section stack taken one-half micron apart. The rightmost image is the middle one deblurred. Applications in Clinical Cytogenetics 823 .

• Đồ họa - Thiết kế - Flash
• thiết kế flash
• thiết kế web
• giáo trình CSS
• thủ thuật cắt html
• photoshop căn bản
• đồ họa máy tính
• Thiết kế đa truyền thông với Adobe Flash CS6
• Adobe Flash CS6
• Chỉnh sửa ảnh Photoshop từ Flash
• Chèn đoạn phim Flash vào Dreamweaver
• Chỉnh sửa đoạn phim Flash từ Dreamweaver
• Nhập file AI từ Illustrator vào Flash
• Tích hợp nội dung Flash
• flash cs5
• thủ thuật flash cs5
• tài liệu flash cs5
• học flash cs5
• ebook flash cs5