TAILIEUCHUNG - HPLC for Pharmaceutical Scientists 2007 (Part 7A)

The discovery of a new drug is a challenging task that includes (a) identifica- tion of a biochemical target for certain diseases and (b) screening of a large number of compounds from libraries of compounds arising from synthetic chemistry, combinatorial chemistry, and natural product isolation for lead gen- eration. The lead compound is then optimized based on biological activity, selectivity, pharmacokinetic property, and metabolism. | 7 LC MS THEORY INSTRUMENTATION AND APPLICATIONS TO SMALL MOLECULES Guodong Chen Li-Kang Zhang and Birendra N. Pramanik INTRODUCTION The discovery of a new drug is a challenging task that includes a identification of a biochemical target for certain diseases and b screening of a large number of compounds from libraries of compounds arising from synthetic chemistry combinatorial chemistry and natural product isolation for lead generation. The lead compound is then optimized based on biological activity selectivity pharmacokinetic property and metabolism. This process produces a large volume of samples requiring rapid and accurate analysis with the speed of analysis contributing directly to the drug discovery cycle time. As one of primer analytical techniques mass spectrometry MS developed from nineteenth-century physics starting with the pioneering work of J. J. Thomson on the electrical discharges in evacuated tubes. In 1913 Thomson wrote I feel sure that there are many problems in Chemistry which could be tackled with far greater ease by this than any other method. The method is surprisingly sensitive more so than that of Spectrum Analysis requires infinitesimal amount of material and does not require this to be specially purified. Indeed MS offers speed high sensitivity and isotopic specificity. This technique separates mixtures of ions on the basis of mass-to-charge ratios HPLC for Pharmaceutical Scientists Edited by Yuri Kazakevich and Rosario LoBrutto Copyright 2007 by John Wiley Sons Inc. 281 282 APPLICATIONS TO SMALL MOLECULES providing the molecular weight of a compound and its structural information from fragment ions. It is widely used for identification and quantification of known unknown organic compounds. Rapid development of MS in recent decades has further expanded its role in the structural characterization of small molecules in the drug discovery process 1 . In combination with chromatographic separation techniques principally in the form of .

• Y học thường thức
• HPLC
• Pharmaceutical Scientists 2007
• HPLC Theory
• tài liệu chuyên ngành dược
• sách học dược sĩ
• pH effect on HPLC separations
• Xác định đồng thời Paracetamol và Ibuprofen
• Phương pháp sắc ký lỏng hiệu năng cao
• Hệ thống sắc ký HPLC
• Hỗn hợp Paracetamol và Ibuprofen
• Hệ thống HPLC
• Phân loại sắc ký hấp phụ
• Bài giảng Các sự cố thường gặp trong phân tích HPLC
• Các sự cố thường gặp trong phân tích HPLC
• Bảo trì hệ thống
• Bảo trì Pump
• Xây dựng quy trình kỹ thuật
• Định lượng Tobramycin
• Nguyên tắc cấu tạo hệ thống HPLC
• Ứng dụng của HPLC
• Qui trình định lượng Tobramycin
• BMC Chemistry
• Preparative HPLC
• Salting out assisted liquid–liquid extraction
• Biomedical sectors
• Catha edulis Forsk
• Fosetyl aluminium
• Fosetyl aluminium fungicide residues
• Garlic using HPLC method
• HPLC method
• High performance chromatographic method
• Xây dựng định lượng cafein
• Phương pháp HPLC định lượng cafein
• Phương pháp HPLC định lượng EGCG
• EGCG trong cao lá Chè xanh
• Cao lá Chè xanh
• Mycophenolic acid
• Validation of a simple HPLC method
• Simple HPLC method
• Quantify mycophenolic acid concentrations
• Human plasma
• Luận văn Thạc sĩ
• Luận văn Thạc sĩ Khoa học
• Hương thảo
• Phương pháp HPLC
• Ứng dụng phương pháp HPLC
• Hóa hữu cơ
• Chromatography A
• Low cost HPLC
• Low cost electrochemical HPLC detector
• Low cost HPLC pump
• Quantified self
• Phương pháp HPLC UV cải tiến
• Quy trình định lượng vitamin C
• Xây dựng đường chuẩn vitamin C
• Sắc kí đồ HPLC
• Đầu dò UV
• chiral separation
• Nitrogen Rule
• method development
• Choice of Stationary Phase
• method development approaches
• effect of pH on UV Absorbance
• stationary phases
• reversed phase HPLC
• ion interaction chromatography