TAILIEUCHUNG - Drugs and Poisons in Humans - A Handbook of Practical Analysis (Part 41)

Introduction: Salicylic acid ( Figure ) is being used as a keratolytic agent for treatment of corns or verrucae; the acid is included in adhesive plasters, ointments and liquid medicines as their ingredient for removing the stratum corneum. Since such products containing salicylic acid are easily obtainable, salicylic acid poisoning (salicylism) cases are relatively many especially for infants not older than 5 years. Its poisoning symptoms were reported to be headache, vertigo, tinnitus, bradyacusia, amblyopia and sweating [1]. For analysis of salicylic acid, HPLC methods were reported [2–7]. In this chapter, a method for HPLC analysis of salicylic acid. | Salicylic acid By Einosuke Tanaka Introduction Salicylic acid Figure is being used as a keratolytic agent for treatment of corns or verrucae the acid is included in adhesive plasters ointments and liquid medicines as their ingredient for removing the stratum corneum. Since such products containing salicylic acid are easily obtainable salicylic acid poisoning salicylism cases are relatively many especially for infants not older than 5 years. Its poisoning symptoms were reported to be headache vertigo tinnitus bradyacusia amblyopia and sweating 1 . For analysis of salicylic acid HPLC methods were reported 2-7 . In this chapter a method for HPLC analysis of salicylic acid in serum specimens is presented 3 . O Figure COOH OH Structure of salicylic acid. Reagents and their preparation 8-Chlorotheophylline internal standard IS Sigma St. Louis MO USA is dissolved in ethanol to prepare 150 pg mL solution. Salicylic acid Sigma is dissolved in methanol to prepare 5-500 pg mL solutions. A aliquot of sodium acetate is dissolved in 1 L water followed by addition of 15 mL acetic acid to prepare acetate buffer solution pH . HPLC conditions Column a reversed phase column pBondapak C18 Radial-Pak 100 x 5 mm . particle diameter 10 pm Waters Milford MA USA . Mobile phase acetate buffer solution pH methanol 72 28 v v . Detection wavelengthb 280 nm flow rate 1 mL min column oven temperature room temperature. Springer-Verlag Berlin Heidelberg 2005 392 Salicylic acid Procedure i. A 100-ph volume of serum 20 ph IS solution and 200 ph of 1 M hydrochloric acid solution are placed in a glass centrifuge tube with a ground-in stopper and mixed well. ii. A 2-mL volume of dichloromethane isopropanol 9 1 v v is added to the above mixture. iii. After mixing or shaking for 5 min it is centrifuged at 2 500 g for 2 min. iv. The organic layer lower phase is transferred to a clear test tube. v. The organic extract is evaporated to dryness under a gentle stream of air or

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