TAILIEUCHUNG - In vitro regeneration of capsicum (Capsicum annuum L.) from cotyledon explants

Pre-requisite for any plant biotechnological approach is development of an efficient plant regeneration system in that crop. In this regard the in vitro regeneration was achieved by using cotyledon explants from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra. Seeds of both hybrids were exposed to different treatments for proper germination, were decontaminated and sown in vitro on half-strength MS medium. The seed soaked in distilled water along with 2 mg/l GA3 for two days prior to sowing had more pronounced effect on both capsicum hybrids recording maximum germination ( and %, respectively) in minimum number of days ( days and days, respectively). | 2017 6 5 225-237 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 6 Number 5 2017 pp. 225-237 Journal homepage http Original Research Article https In Vitro Regeneration of Capsicum Capsicum annuum L. from Cotyledon Explants Vivek Hegde1 3 . Partap1 and . Yadav2 1 Department of Vegetable Science CCS Haryana Agricultural University Hisar-125 004 Haryana India 2 Department of Molecular Biology and Biotechnology CCS Haryana Agricultural University Hisar-125 004 Haryana India 3 Division of Crop Improvement ICAR- Central Tuber Crops Institute Thiruvananthapuram Kerala India Corresponding author ABSTRACT Pre-requisite for any plant biotechnological approach is development of an efficient plant regeneration system in that crop. In this regard the in vitro regeneration was achieved by Keywords using cotyledon explants from aseptically raised seedlings of popular Capsicum F 1 hybrids Bharat and Indra. Seeds of both hybrids were exposed to different treatments for proper In vitro germination were decontaminated and sown in vitro on half-strength MS medium. The regeneration seed soaked in distilled water along with 2 mg l GA3 for two days prior to sowing had Cotyledon more pronounced effect on both capsicum hybrids recording maximum germination Capsicum and respectively in minimum number of days days and days Tissue culture. respectively . Regeneration potential of cotyledonary explants touching the medium both Article Info abaxial and adaxial sides was examined. Tissue culture responses varied with the genotypes side of explants touching the medium and combinations of growth regulators Accepted used. Regeneration per cent number of shoots per explants and per cent 04 April 2017 shoot elongation was maximum in hybrid Indra from cotyledon explant having Available Online abaxial side in contact with growing .

• Nông nghiệp
• In vitro regeneration
• Tissue culture
• In vitro regeneration of capsicum
• Cotyledon explants
• Popular Capsicum F1 hybrids Bharat
• Tinospora cordifolia
• Hormone on in vitro regeneration
• Genetically manipulated superior clones
• Plant micropropagation
• Gerbera jamesonii Bolus
• Direct in vitro regeneration
• In vitro regeneration of gerbera
• Gerbera jamesonii
• BAP 6 benzyl amino purine
• In vitro micro propagation regeneration
• Chickpea plant
• Regeneration of chickpea plant
• In vitro micro propagation
• plant growth regulators NAA
• Journal of Agriculture and Forestry
• Culture media
• Plant regeneration
• Vitro regeneration protocol
• Dactylis glomerata L
• In vitro mutation studies
• In vitro
• Woody plant media
• 2 isopentinyl adenine
• Indole 3 acetic acid
• In vitro studies on plant regeneration
• Elite clones of cocoa
• Elephant foot yam
• Surface sterilization
• In vitro techniques
• Culture establishment
• In vitro regeneration technique
• Multiple shoot regeneration
• Plumular apices of chickpea
• Shoot regeneration
• Genetic transformation
• Brinjal variety KKM1 seeds
• Callus induction
• Indirect organogenesis for regeneration
• Regeneration in brinjal
• Hypocotyl of in vitro grown brinjal
• Hypocotyl explants in capsicum
• Hypocotyl explants of Capsicum hybrids Bharat
• Growth regulators on soybean
• Soybean regeneration
• Cotyladory node explants were taken
• In vitro regeneration of Soybean cultivars
• Soybean cultivars
• Naga chilli
• In vitro propagation
• Silver nitrate
• Naga chilli (Capsicum chinense Jacq )
• Callus mediated in vitro regeneration
• Anthemis xylopoda
• Endemic plant
• Adventitious shoot regeneration
• In vitro flowering
• Indole3 acetic acid
• Endemic species
• Immature embryo
• Iris sari
• Iris schachtii
• Linum usitatissimum
• hypocotyl explants
• In vitro competition
• Explants cultured
• Citrus species
• Callus induction and regeneration
• Citrus plant breeding of rootstocks
• Recalcitrance of citrus seeds
• Apple cultivar
• Produced axillary
• Supplemented with BA
• MS medium augmented with BA
• Vigna radiata
• Immature cotyledons
• Genetic improvement
• Recalcitrant green gram
• Variety Nanjanagudu rasabale
• IBA Accepted
• Efficient protocol
• Producing better roots
• Mature seed
• Somatic embryogenesis
• Maize inbreds using mature seeds
• Embryos of maize inbred
• Mature embryo
• MS medium
• Proso millet (Panicum miliaceum L )
• Mature embryo of proso millet
• BMC Plant Biology
• In vitro plant regeneration
• Daylilies tissue culture
• Shoot organogenesis
• Nutritional value
• Gladiolus hybrida
• Growth regulators
• RAPD markers
• gladiolus (Gladiolus hybrida L )
• Multi walled carbon nano tubes
• Functionalized carbon nano tubes
• Malbhog banana
• Plant growth regulators
• Callus culture
• Leaf explant
• Parthenocarpic cucumber
• Media compositions exhibited varying responses