TAILIEUCHUNG - Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract

Abstract This study was conducted in order to evaluate the probiotic properties of lactic acid bacteria (LAB) isolated from intestinal tract of broilers and Thai indigenous chickens. The major properties, including the gastric juice and bile salts tolerance, starch, protein and lipid digesting capabilities, and the inhibition on certain pathogenic bacteria were investigated. Three-hundred and twenty-two and 226 LAB strains were isolated from ten broilers and eight Thai indigenous chickens, respectively | World J Microbiol Biotechnol 2009 25 1337-1345 DOI s11274-009-0020-8 ORIGINAL PAPER Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract H. Musikasang A. Tani A. H-kittikun S. Maneerat Received 28 October 2008 Accepted 16 March 2009 Published online 31 March 2009 Springer Science Business Media . 2009 Abstract This study was conducted in order to evaluate the probiotic properties of lactic acid bacteria LAB isolated from intestinal tract of broilers and Thai indigenous chickens. The major properties including the gastric juice and bile salts tolerance starch protein and lipid digesting capabilities and the inhibition on certain pathogenic bacteria were investigated. Three-hundred and twenty-two and 226 LAB strains were isolated from ten broilers and eight Thai indigenous chickens respectively. The gastrointestinal transit tolerance of these 548 isolates was determined by exposing washed cell suspension at 41 C to simulated gastric juice pH containing pepsin 3 mg ml-1 and to simulated small intestinal juice pH in the presence of pancreatin 1 mg ml-1 and 7 fresh chicken bile mimicking the gastrointestinal environment. The survival of 20 isolates was found after passing through the gastrointestinal conditions. The survival rates of six strains KT3L20 KT2CR5 KT10L22 KT5S19 KT4S13 and PM1L12 from the sequential study were and respectively. Twelve isolates exhibited protein digestion on agar plate but no isolates showed the ability to digest starch and lipid. All 20 LAB showed the antimicrobial activity against Salmonella sp. Staphylococcus aureus and Escherichia coli except one strain which did not show the inhibitory activity toward E. coli. Accordingly five isolates of selected LAB KT2L24 KT3L20 KT4S13 KT3CE27 H. Musikasang A. H-kittikun S. Maneerat El Department of Industrial Biotechnology Faculty of Agro-Industry Prince of Songkla University Hat Yai 90112 Thailand .

TỪ KHÓA LIÊN QUAN
TAILIEUCHUNG - Chia sẻ tài liệu không giới hạn
Địa chỉ : 444 Hoang Hoa Tham, Hanoi, Viet Nam
Website : tailieuchung.com
Email : tailieuchung20@gmail.com
Tailieuchung.com là thư viện tài liệu trực tuyến, nơi chia sẽ trao đổi hàng triệu tài liệu như luận văn đồ án, sách, giáo trình, đề thi.
Chúng tôi không chịu trách nhiệm liên quan đến các vấn đề bản quyền nội dung tài liệu được thành viên tự nguyện đăng tải lên, nếu phát hiện thấy tài liệu xấu hoặc tài liệu có bản quyền xin hãy email cho chúng tôi.
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.