TAILIEUCHUNG - Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs

The inhibitory activity of these spore-forming strains against Escherichia coli K88, E. coli K99, Salmonella typhimurium and Staphylococcus aureus was assessed using a disc plate diffusion assay. The six bacilli with the largest inhibition zones against the four indicator bacteria were chosen and assessed for their | Antonie van Leeuwenhoek 2006 90 139-146 DOI s10482-006-9067-9 ORIGINAL PAPER Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtìlỉs MA139 in pigs Xiaohua Guo Defa Li Wenqing Lu Xiangshu Piao Xiaoling Chen Received 13 December 2005 Accepted 3 March 2006 Published online 4 July 2006 Springer Science Business Media . 2006 Abstract A total of 124 samples were collected from the intestine of broiler chickens piglet faeces fermented foods soils and Chinese herbs. More than 750 strains of aerobic spore-forming bacteria were isolated from these samples. The inhibitory activity of these spore-forming strains against Escherichia coli K88 E. coli K99 Salmonella typhimurium and Staphylococcus aureus was assessed using a disc plate diffusion assay. The six bacilli with the largest inhibition zones against the four indicator bacteria were chosen and assessed for their resistance to unfavorable conditions within simulated gut environments. The strain Bacillus subtilis MA139 showed full resistance to pH 2 bile salts and exhibited the highest antimicrobial activity. Based on these results B. subtilis MA139 was selected as a potential probiotic and fed to piglets at concentrations of X 105 X 106 or 2 2 X 107 CFU g of feed during a 28-day feeding trial. A negative control consisting of the basal diet with no additives and a positive control consisting of the basal diet supplemented with 16 g ton flavomycin were also included. Ninety piglets between 35 and 40 days old were used X. Guo D. Li W. Lu X. Piao X. Chen National Key Laboratory on Animal Nutrition China Agricultural University No. 2. West Road Yuanmingyuan Beijing 100094 . China E-mail defali@ Tel. 8610-62733588 Fax 8610-62733688 in the in vivo animal trials. B. subtilis MA139 enhanced daily gain P and feed conversion P compared with the negative control. The performance of pigs fed B. subtilis .

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