TAILIEUCHUNG - Selection of oleaginous yeasts for fatty acid production

Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. | Lamers et al. BMC Biotechnology 2016 16 45 DOI s12896-016-0276-7 BMC Biotechnology RESEARCH ARTICLE Open Access Selection of oleaginous yeasts for fatty acid production r-x I 1 7 1 K 1 rv 14 r-x1 1 A A 9 I I r . 1 I 1 7 1 1 Dennis Lamers1 2 T Nick van Biezen Dirk Martens2 Linda Peters Eric van de Zilver Nicole Jacobs-van Dreumel1 René H. Wijffels2 3 and Christien Lokman1 Abstract Background Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides TAGs . These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 to 70 of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-called second generation biofuels which are based on non-food competing waste carbon sources . Results In this study the selection of potentially new interesting oleaginous yeast strains is described. Important selection criteria were a broad maximum temperature and pH range for growth robustness of the strain a broad spectrum of carbon sources that can be metabolized preferably including C-5 sugars a high total fatty acid content in combination with a low glycogen content and genetic accessibility. Conclusions Based on these selection criteria among 24 screened species Schwanniomyces occidentalis Debaromyces occidentalis CBS2864 was selected as a promising strain for the production of high amounts of lipids. Keywords Oleaginous yeast Schwanniomyces occidentalis Lipid production TAG Background Mineral and vegetable oil is a crucial resource for the modern human civilization but the worldwide amount is depleting rapidly and alternatives need to be explored. An interesting alternative to decrease the dependency of western societies on these fossil and vegetable sources might be the use of oleaginous micro-organisms as described by various authors 1-3 . Lipids isolated from oleaginous micro-organisms can be used as components in coatings paints personal care products production .

• Sinh học
• BMC Biotechnology
• Oleaginous yeast
• Schwanniomyces occidentalis
• Lipid production
• Rhodosporidium toruloides
• Cryptococcus curvatus
• Transgenic animals
• Recombinant proteins
• Animal biotechnology
• Functional cytokines
• ABC transporter
• Taxonomic diversity
• ATP binding cassette
• Transporter gene superfamily
• Community engagement
• Agricultural biotechnology
• Tissue culture
• Global health
• Global development
• Stakeholder engagement
• BMC Chemistry
• Citric acid
• White biotechnology
• Organic acid
• Emerging bioeconomy
• MicroRNAs
• cDNA synthesis
• synthetic templates
• Design of PCR primers
• Quantitative PCR
• Error prone PCR
• Site directed mutation
• Site saturation mutagenesis
• Catalytic efficiency
• Tobacco stalk
• Nicotine degradation
• Phanerochaete chrysosporium
• Lignocellulolytic enzymes
• Chaetomium globosum
• Synergistic action
• Lignocellulosic materials
• Phenolic alcohols
• β xylosidase
• Highly thermostable GH39
• Geobacillus sp
• Hydrolyzing xylo oligosaccharides
• Heterologous expression
• Kluyveromyces lactis
• Baking industry
• Plectosphaerella cucumerina
• Gelatin scaffold
• Human articular chondrocytes
• Extracellular matrix
• Cartilage tissue engineering
• Protein design
• Protein expression
• Protein interaction
• Protein engineering
• Pseudomonas putida strains
• Raw glycerol
• PHA depolymerase
• Metabolic engineering
• Protein cross linking
• Protein stability
• Baculovirus expression vector system
• Human influenza strain
• Arteriovenous loop
• PHD inhibitor
• Dimethyloxallyl Glycine
• HIF α level
• Substitution pattern
• Hydroxypropyl methylcellulose
• Application of carbohydrate binding modules
• Hydroxyethyl cellulose
• Rhizomucor pusillus
• α amylase
• Co expression
• Pichia pastoris
• Alkaline phosphatase
• Escherichia coli
• Psychrophilic alkaline phosphatase
• Hydrolytic cleavage
• Leuconostoc citreum
• Inulosucrase IslA
• Multidomain fructosyltransferase
• Multidomain enzyme
• Hydrolytic activity
• CD spectroscopy
• Homology modelling
• Peroxidase activity
• Superoxide dismutase
• Trichoderma reesei
• Endoglucanase I
• Cell free synthesis
• Saccharomyces cerevisiae
• Neurospora crassa
• Glutaminyl cylcase
• Tobacco mosaic virus
• Protein purification
• N benthamiana
• Active pharmaceutical ingredient
• Adenoviral vector
• Inducible vector
• Gene therapy
• Optimizing therapeutic outcomes
• Co transformation
• Gaussia princeps luciferase gene
• Genetic engineering
• Green algae
• Inducible promoter
• Nitrate reductase
• Spider silk
• Synthetic spidroin
• Atomic force microscopy
• Outstanding mechanical