TAILIEUCHUNG - Antibiotic sensitivity pattern and characterization of staphylococcus aureus for thermonuclease gene (nuc) in bovine affected with mastitis from cauvery delta region of Tamilnadu, India

Among the various mastitis pathogens, Staphylococcus aureus (S. aureus) is identified as a chief etiological agent responsible for subclinical and chronic mastitis. Samples were collected from the clinically affected cattle showing typical symptoms of mastitis. 156 samples were screened by clinical symptoms specific for mastitis from the delta region of Tamilnadu. On the basis of cultural and biochemical properties, 52 isolates were presumptively identified as S. aureus. These 52 isolates were subjected to antibiotic sensitivity test, for which 15 antibiotic discs were used. The antibiotic sensitivity pattern of the used antibiotics of the 52 Staphylococcal isolates revealed maximum resistance for pencillin-G (90%), ampicillin (83%), erythromycin (60%), terramycin (54%), Ampicillin/ Cloxacillin (47%) and cotrimaxazole (46%). whereas, the isolates were highly sensitive to chloramphenical (84%), enrofloxacin (75%), ofloxacin (61%), ceftriaxone (45%), and amoxicillin + clavulanic acid (50%). All the isolates were susceptible to Vancomycin (100%). Intermediate pattern was observed in ceftrioxone (14%), ofloxacin (40%) and gentamicin (30%). These 52 isolates were genotypically identified as S. aureus by Polymerase Chain Reaction (PCR) for species specific thermonuclease gene (nuc). Amplification for thermonuclease (nuc) gene was observed in 52 solates. The amplified products were of nearly 279 bp when resolved in gel electrophoresis. The phenotypic and genotypic findings of the present study might help to understand the distribution of prevalent S. aureus infection in the dairy farms and antibiotic sensitivity pattern will help to choose most appropriate antibiotic, hence help to adopt appropriate strategies for the management and control of bovine mastitis. | Antibiotic sensitivity pattern and characterization of staphylococcus aureus for thermonuclease gene (nuc) in bovine affected with mastitis from cauvery delta region of Tamilnadu, India

• Sinh học
• Bovine mastitis
• Staphylococcus aureus
• Antibiotic resistance
• Mastitis from cauvery delta region
• Characterization of staphylococcus aureus
• Bovine clinical mastitis caused
• Escherichia coli
• Polymerase chain reaction
• Clinical mastitis
• Mastitis in cows
• Chronic mastitis
• Factors affecting mastitis
• Fishers linear discriminant function
• Classification of bovine mastitis
• PCR and sequencing
• Methicillin resistant coagulase
• Genotypic identification
• Bovine clinical mastitis
• Antibiogram of the isolates
• Isolates in Odisha
• Morphological and biochemical characteristics
• Prophylactic effects
• Turmeric against bovine mastitis
• Prophylactic effects of turmeric
• Criteria of selection
• Supplementation of anti oxidant
• Mineral formulation
• DCC in bovine mastitis
• Milk SCC in bovine mastitis
• Anti oxidant mineral formulation
• Supplementation of anti oxidant mineral formulation
• Recombinant human lipocalin 2
• PIRES2 vector
• Bovine mammary epithelial stem cells
• Mammalian specific
• BMC Veterinary Research
• Cytokine profile
• In vitro model
• Precision cut bovine udder slices
• Methicillin resistant Staphylococcus aureus isolated
• Genotypic methods
• Main cause of bovine mastitis
• Pre milking teat disinfection
• Post milking teat disinfection
• Mastitis levels
• Organized dairy farm
• Klebsiella pneumoniae
• Bovine mammary epithelial cells
• Bovine teat
• Tea extract
• Antimicrobial activity
• Camellia sinensis
• Various tea extracts
• Dairy cattle
• Oxidative stress
• Bovine mastitis related oxidative stress
• Therapeutic regimen
• Multi etiological complex disease
• Somatic cell count
• Biomarker for early diagnosis
• Therapeutic evaluation
• Isolation of E coli
• Antibiotic sensitivity pattern
• Antibacterial agents
• Bacterial isolates
• In vitro antibiotic sensitivity pattern
• Coagulase negative Staphylococci isolated
• Costly problem of dairy cattle
• Virulent gene characterization
• Methicillin sensitive staphylococcus aureus
• Methicillin resistant staphylococcus aureus
• Staphylococcal enterotoxin genes
• Relative expression of TLR9 gene
• TLR9 gene in natural sub clinical
• Clinical cases
• Clinical cases of bovine mastitis
• Therapeutic efficacies
• Self formulated antioxidant
• Self formulated anti oxidant trace minerals formulation
• Mastitic animals
• Potassium permanganate
• Antiseptic efficacy and milk
• Control of bovine mastitis
• Potassium permanganate as antiseptic agent
• Testing the efficacy of potassium permanganate
• Pyruvate formate lyase gene
• Immunogenic protein
• Major streptococcus spp
• Molecular detection
• Antimicrobial resistance
• Antimicrobial resistance pattern
• Biochemical characterization
• Bovine mastitic milk
• Biochemical characterization of escherichia coli
• Antibacterial effect
• Comparative in vitro antibacterial efficacy
• Common herbs
• Antibiotics against