TAILIEUCHUNG - Morphological and molecular characterization of Marssonina coronaria (Ellis & J.J. Davis) J.J. Davis causing premature leaf fall

Marssonina leaf blotch caused by the fungus Marssonina coronaria (Ellis & . Davis) . Davis causing premature leaf fall is one of the major economic constraint which hampers apple production in India. Seven isolates of Marssonina coronaria were isolated and evaluated for their morphological and molecular characterization using ITS primers. All the seven isolates were identified based on the morphological characteristics, and produced brown to black colonies without aerial mycelium, formed a wrinkled surface, on PDA after incubation for 30 days. Conidia are ampule shaped, one sepate, constricted at the septum, hyaline, and guttulate. However, not much difference was obtained in colony diameter and size of conidia. Seven isolates of the rDNA of ITS region was amplified by ITS4/ITS6 primers and ranged from 565~615 bp in the isolates. On BLAST, isolates showed high homologies (96~100%) with Marssonina coronaria. Phylogentic tree depicted that the isolates were closely clustered with Chinese isolates with accession numbers FJ606800, EU329733, EU329735, FJ618562, FJ618561 and FJ618560. The present study demonstrated the genetic relationships among the isolates of Marssonina coronaria and indicated that ITS rDNA sequence data were potentially useful in taxonomic species determination. | Morphological and molecular characterization of Marssonina coronaria (Ellis & . Davis) . Davis causing premature leaf fall

• Sinh học
• Marssonina coronaria
• ITS regions
• Premature leaf fall
• Molecular characterization
• Morphological and molecular characterization
• Morphological characterization
• Aromatic farmer’s rice varieties collected
• Different districts
• Aromatic farmer’s rice
• Molecular and morphological characterization
• Magnaporthe grisea
• Bacillus strains against M grisea
• Magnaporthe grisea and bio efficacy
• Samba mahsuri
• Complete panicle emergence
• Morphological traits
• Genetic relatedness
• Complete panicle emergence mutant lines
• Oyster mushroom
• Molecular characterization of oyster mushroom
• Subtropical regions of the world
• Macro fungus with a distinctive fruiting body
• Fusarium wilt
• Bitter gourd
• Fusarium wilt of bitter gourd
• Genetic diversity
• Morphological markers
• SSR markers
• Molecular descriptors
• Varieties using morphological
• Purple blotch
• Purple blotch disease of onion
• Alternaria isolates
• Maize ear rot
• Species specific primers
• Fusarium verticillioides
• Cultural of Fusarium verticillioides
• Near isogenic lines
• Rice (Oryza sativa L
• Major abiotic stresses of rice
• Near isogenic lines developed
• Coconut nut rot
• Lasiodiplodia theobromae
• Scanning electron microscope
• Inoculation techniques
• Sheath blight
• Rhizoctonia solani
• Causing sheath blight in rice
• Haemonchus contortus
• Trichostrongylus colubriformis
• Anthelmintic resistance
• Gastrointestinal nematodes in sheep
• Journal of Botany
• Fodder pea
• Genetic differences
• Simple sequence repeat
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Molecular marker systems
• Random amplified polymorphic DNA
• Sequence related amplified polymorphism
• Unweighted pair group method with arithmetic averages
• Morphological characters
• RAPD marker
• SSR marker
• Dendrobium orchid species
• Internal transcribed spacer
• Light microscopy
• Biogeochemical cycling of silica
• Polymerase chain reaction
• Vanilla planifolia
• Fusarium oxysporum
• Root and stem rot
• ITS region
• Maximum likelihood
• The Journal of Agriculture and Development
• Tạp chí Nông nghiệp và Phát triển
• IAA production
• Phosphorus solubilization
• Salt tolerant bacteria
• High salt environments naturally
• Turkish Journal of Agriculture and Forestry
• Tạp chí Nông Lâm nghiệp
• Amplified fragment length polymorphism
• Bayesian cluster analysis
• Principal component analysis
• Croatian carob tree