TAILIEUCHUNG - Cloning, expression and in silico characterization of a truncated antiviral protein gene from Bougainvillea spectabilis willd.

Bougainvillea antiviral protein (BAP) is one among a class of the ribosomal inactivating proteins isolated from Bougainvillea spectabilis willd. Truncated version of the BAP gene was cloned and expressed in a prokaryotic vector to abolish its cytotoxicity. RNA was isolated from mature leaves of Bougainvillea and the full length cDNA was amplified by reverse transcription-PCR using template mRNA. This full length cDNA of size 756 bp was amplified using the proofreading polymerase (Q5 polymerase) and end to end gene specific primers for removal of C-terminal, the amplicon was cloned in vector by blunt end cloning method. Restriction digestion was performed to release the fragment which was further ligated into prokaryotic expression vector pET29a. The recombinant plasmid was transferred into expression strain BL21 (DE3) and the truncated-BAP gene was expressed by isopropyl β-D thiogalactopyranoside (IPTG) induction. Transformed colonies expressed recombinant fusion Bougainvillea antiviral protein of molecular weight ~ kDa, the size expected for the truncated BAP gene. | Cloning, expression and in silico characterization of a truncated antiviral protein gene from Bougainvillea spectabilis willd.

• Sinh học
• Bougainvillea spectabilis Willd
• Q5 Polymerase
• Truncated antiviral protein gene
• Bougainvillea spectabilis willd
• Silico characterization
• Salmonella typhimurium
• In silico analysis
• Immune potential
• Sequencing and in silico characterization
• OmpF protein
• Resistant gene
• In silico
• Disease resistance gene analogues
• Minor Millets
• Biotechnology and food sciences
• In silico characterization
• Glucanase promoter from drosera binata
• Cis acting elements
• Drosera binata
• MD simulation
• Molecular dynamics simulation
• Expression analysis
• Class I chitinases
• Aloe barbadensis
• Pigeon pea
• Gene expression
• Pigeon pea (Cajanus cajan L )
• Expressed sequence tags
• Characterization of conserved miRNAs
• Tomato leaf curl virus
• Whiteflies and phylogenetic relationship
• Phylogenetic relationship
• Tomato leaf curl virus associated
• Early node
• Chromosomal mapping
• Functional annotation
• Homology modeling
• Wheat using in silico approach
• Expression profiles
• Sucrose transporter
• Gene family in peanut
• Structural characterization
• Sucrose transporter ‘SWEET’ gene family
• Solanum lycopersicum
• Proteinase inhibitor
• Wound inducible
• In vitro
• Phylogenetic tree
• Teratodon nigrovirdis
• Salmo salar
• Chromatophores Bioinformatics
• Virtual screening
• BMC Genomics
• M abscessus subspecies
• Non tuberculous mycobacterium
• M tuberculosis complex
• Survival inside
• Somatic embryogenesis
• Somatic tissue
• Growth regulators
• Phylogenetic analysis
• Arabidopsis thaliana proteins
• Leishmania braziliensis
• Virulence proteins
• Genus Leishmania
• Highly divergent eukaryotes
• Biological features
• Energy taxis
• Oxygen sensing
• Signal transduction
• C coralloides
• Chemotaxis proteins
• Journal of Chemistry
• Antibacterial activity
• FT IR spectral foundations
• Silico chemoinformatic
• Antimicrobial drugs
• Cytotoxic activity