TAILIEUCHUNG - Comparison of conventional culture and reverse transcriptase real time PCR assay for detection of tuberculosis in sputum samples

Tuberculosis remains a major public health threat worldwide. Detection of tuberculosis by culture is greatly hampered by long turn-around times up to four weeks. Early detection of tuberculosis is important to initiate anti TB drug regimen at the earliest. A step forward is the ribosomal 16SrRNA Real-time Reverse Transcriptase PCR (qRT- PCR) which provides rapid results with high accuracy, sensitivity and specificity. The present study compares the conventional culture and qRT-PCR using ribosomal 16SrRNA for detection of M. tuberculosis in sputum samples of TB patients. RNA was extracted from 211 sputa, then qRT PCR was conducted. All samples were cultured on Lowenstein-Jensen medium after processing by NALC-NaOH method. Of 211 samples, 66 () were positive and 145 () were negative by qRT PCR. Of 66 positive samples, 54 () were culture positive and 12 () were culture negative. Of 145 negative samples, three were culture positive and 142 () were culture negative. Our RT PCR assay had % accuracy having positive predictive value of and negative predictive value of %, with high sensitivity ( %) and specificity (). The qRT PCR may be included in the diagnostic algorithm for early detection of MTB in TB patients. | Comparison of conventional culture and reverse transcriptase real time PCR assay for detection of tuberculosis in sputum samples

• Y khoa - Dược
• Reverse transcriptase
• Sputum samples
• Tuberculosis in sputum samples
• Comparison of conventional culture
• Reverse transcriptase real time PCR
• Gastrointestinal cancer
• Reverse transcriptase PCR
• Gastrointestinal malignancies
• Cytokeratin 19
• Human telomerase reverse transcriptase
• Reverse transcriptase polymerase chain
• Luận văn Thạc sĩ Khoa học
• Telomerase reverse transcriptase
• Hệ nano chitosan
• Vector tái tổ hợp
• Kháng nguyên telomerase reverse transcriptase
• Sinh học thực nghiệm
• Journal of Chemistry
• Quinoline moiety
• Non nucleoside reverse transcriptase inhibitors (NNRTIs)
• Transcriptase binding
• Quinoline derivatives
• BMC Bioinformatics
• Artificial neural network
• Drug resistance prediction
• Subtype specific training
• HIV 1 subtype B
• HIV reverse transcriptase
• HIV protease
• Hybrid structure modeling
• Molecular dynamics
• Solvated interaction energy
• Hepatitis B
• Drug resistance
• Infectious bronchitis
• Respiratory pathogens
• Infectious bronchitis virus
• Chicken manifesting respiratory signs
• Transcriptase polymerase chain reaction
• Scientific reports
• scientific research
• Structural biochemistry
• DNA polymerase
• medical analysis
• environmental medicine
• trình bày báo cáo
• tài liệu báo cáo khoa học
• nghiên cứu y học
• báo cáo y học
• kiến thức y học
• Whole transcriptome analysis
• Ribosomal RNAs
• rRNA removal
• Reverse transcription
• Next generation sequencing
• báo cáo khoa học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• hospitalized patients
• cohorts suggesting
• HIV transmission
• directly attrib
• inhibitors (NRTI)
• tuberculosis typically
• extra pulmonary
• malnutrition