TAILIEUCHUNG - Cloning of GFP Tagged MYB-6 Gene: An important transcription factor in regulating Anthocyanin biosynthesis of Daucus Carota

MYB-6 gene modulates the biosynthetic pathway of anthocyanins in plants in response to cold stress. In the present study, the full length version of this gene was identified and characterized from black carrot (Daucus carota L.) through PCR amplification using specific primers. The size of amplified MYB-6 gene was found to be 903bp which was confirmed through sequencing. Post double digestion of both vector (pEGFP-C1) and Insert (MYB-6 gene), the ligated product was subjected to transformation using bacterial host ( DH5ɑ). Confirmation of successful transformation has revealed no growth of cells on Kanamycin enriched LB-plates, while as clear colonies were found on vector and vector-insert LB-plates. Further, analysis via PCR, restriction digestion and gene sequencing has confirmed successful cloning of carrot derived MYB-6 gene in DH5ɑ. In the current study, we aimed to clone GFP tagged MYB-6 gene that could act as easy to use gene pool candidate for amelioration of cold susceptible crops and for sustainable agricultural development through various high-throughput transgenic studies. The GFP tagged MYB-6 can be used for localization studies as well. | Cloning of GFP Tagged MYB-6 Gene: An important transcription factor in regulating Anthocyanin biosynthesis of Daucus Carota

• Sinh học
• Daucus carota
• MYB 6 and Restriction digestion
• Regulating Anthocyanin biosynthesis
• GFP Tagged MYB 6 Gene
• Aimed to clone GFP
• Nitrate accumulation
• Daucus carota L
• Carrot Daucus carota L
• plants
• Total N concentration
• Nitrogen fertilization
• Excess boron
• Root cells
• Daucus carota cv
• Nantes
• Scanning electron microscopic
• Coefficients and genotypes
• Carrot genotypes (Daucus carota L )
• Genotypic correlation coefficient analysis
• Different characters in carrot genotypes
• Genotypic coefficient of variation
• Phenotypic coefficient of variation
• Genetic advance over mean and genotypes
• Ducus carota L
• Osmotic dehydration
• Physical parameters
• Osmotically dehydrated carrot (Daucus carota L )
• Organoleptic properties
• In vitro anther cultures of carrot
• Kazan F1
• Narbonne F1
• Glucose 6 phosphate isomerase
• Minimal processing
• Carboxy methyl cellulose
• Sensory scores
• Carrots (Daucus carota)
• Physico chemical composition
• Seed morpho metric
• Physiological tests
• Biochemical tests
• Biochemical characterization on carrot
• Seed yield and RSC water
• Influence of fym and gypsum
• Gypsum on growth
• Seed yield in carrot
• Irrigated with high RSC water
• Chất lượng của cà rốt
• Chế phẩm vi khuẩn lactobacilus acidophilus
• Quá trình chần nguyên liệu
• Quy trình lên men cà rốt
• BMC Plant Biology
• Anthocyanin pathway
• Root development
• Purple carrot
• Gene expression
• Inorganic fertilizer
• Quality of carrot
• Integrated nutrient management
• Pay significant price premiums
• BMC Genomics
• RNA Seq
• Differential expression analyses
• Transcription factors
• Gene collinearity
• Genomic fractionation
• Daucus carota subsp carota L
• Paleo polyploidization
• Độc tố vi khuẩn lam
• Ảnh hưởng của độc tố vi khuẩn lam
• Sự nảy mầm của cà rốt
• Sự sinh trưởng của cà rốt
• Sự nảy mầm của xà lách
• Sự sinh trưởng của xà lách
• Tạp chí phân tích Lý Hóa Sinh
• Hàm lượng Cu2⁺
• Hàm lượng Zn2⁺
• Cây rau xà lách mỡ
• Cây cà rốt
• Nền đất ô nhiễm kim loại nặng
• Khả năng sinh trưởng cà rốt
• Phát triển cà rốt
• Năng suất cà rốt
• Thừa Thiên Huế
• Giống cà rốt
• Nghiên cứu bổ sung
• Bổ sung cà rốt
• Thức ăn lên sinh trưởng
• Chất lượng tôm thẻ chân trắng
• Nuôi theo công nghệ biofloc
• Integrated farming
• Organic farming
• Phenolic acid
• Sugar and acid components
• Genetic variation
• SSR marker
• Structure analysis
• Carrot variety
• Sowing date and yield
• Varying sowing times
• Chemical compositions
• Different varieties of carrot
• Respect yield
• Phân bón kali
• Chất lượng cà rốt
• Năng suất củ cao
• Công thức bón kali
• Root traits genetic variability
• Genetic advance
• European type carrot germplasm lines
• Economic root traits in asiatic
• Heritability and genetic advance
• Transcript profiles
• Hormonal crosstalk
• Undergoes obvious alteration