TAILIEUCHUNG - Psp68, a dead box helicase confers salinity tolerance in transgenic pigeon pea

Legumes are an important part of human diet account for about 27% of global primary crop production. Pigeon pea is the world’s sixth most important and second most important legume pulse crop of India after chickpea and mainly cultivated as rain fed crop. Its production is adversely affected due to salinity in arid and semi-arid regions of world. Salt stress reduces water potential, creates imbalance in ion concentration and causes toxicity. Helicases have been shown to play an important role in plants against salt stress. p68 which is a prototype member of DEAD-box helicase interacts with Ca2+ -CaM, thus regulating diverse signalling pathways against salt stress in plants. In the present study, we have developed transgenic pigeon pea plants with marker free gene Psp68 for salinity tolerance. Since regeneration is prerequisite for transgenic development and pigeon pea is considered to be recalcitrant, the transgenic pigeon pea plants containing Psp68 gene have been developed using the tissue culture independent transformation method (Patent Application No. 201811012099). | Psp68, a dead box helicase confers salinity tolerance in transgenic pigeon pea

• Sinh học
• Agrobacterium transformation
• Physiobiochemical analysis
• Transgenic pigeon pea
• Confers salinity tolerance
• Dead box helicase confers salinity
• BMC Plant Biology
• Agrobacterium tumefaciens transformation
• Gene editing
• Agrobacterium mediated genetic transformation
• Biolistic transformation
• Genetic transformation
• Squirting cucumber
• Agrobacterium tumefaciens
• Mediated transformation of plants
• PAoPR1 GUS INT plasmids
• Agrobacterium mediated transformation of grapefruit
• Citrus tristeza virus
• Plant transformation
• Replicase mediated resistance
• RNA dependent RNA polymerase
• Efficient protocol for Agrobacterium mediated transformation
• Gus gusplus gene into cassava plants
• Manihot esculenta Crantz
• Tumefaciens bacterial strains
• Three antibiotics kanamycin
• Solanum trilobatum L
• Direct shoot organogenesis
• Histochemical gus analysis
• RNA interference
• Arabidopsis thaliana
• Binary vector
• Aromatic indica rices
• Immature embryos
• Aromatic Indica rices mediated
• Agrobacterium mediated transformation
• Citrus postharvest pathogen
• DsRed fluorescent reporter gene
• Penicillium digitatum
• Phleomycin resistance marker
• BY 2 cells
• Agrobacterium tumefaciens mediated transformation
• In vitro culture
• GUS transient expression
• Chemical and biological
• Plant Biology
• Hairy root transformation
• Composite plants
• Agrobacterium rhizogenes
• Solanum lycopersicum
• Sinorhizobium fredii
• BMC Biotechnology
• Agrobacterium infiltration
• Gene expression
• Phaseolus vulgaris
• Transient transformation
• Back gram
• Black gram (Vigna mungo L )
• Cultivar VBN 3
• Agrobacterium mediated
• Planta genetic transformation
• Verticillium dahliae
• PEG mediated transformation
• Short interfering RNAs
• Brittle 2
• Transformation frequency
• Transgenic plant
• Endosperm starch biosynthesis
• Gene transformation
• Hairy roots
• Panax vietnamensis Ha et Grushv
• PCR amplification
• Electroporation mediated transformation
• Chlamydomonas reinhardtii
• Tạp chí khoa học
• Tạp chí sinh học
• Manihot esculenta
• Gene modification
• Transient expression
• Syringe Agrobacterium infiltration
• Transgenic poplar
• Secondary wall formation
• Echinacea purpurea
• Regeneration
• A
• tumefaciens
• Transformation
• Plant Biotechnology Department
• pruriens
• Yeast extract peptone agar
• Nontransgenic plant DNA
• Polymerase chain reaction
• Generation of transgenic sugar beet
• Phytopathogenic fungi
• Polygalacturonase inhibiting proteins
• Multiple gene transformation
• Implications in plant breeding
• MGT has applications
• Molecular pharming
• Flavanone 3 hydroxylase
• Dendrobium orchid
• Protocorm like bodies
• Gateway cloning system
• Fluorescence reporter
• Transcript fusion
• Agrobacterium tumifaciens
• Shoot apex
• β glucoronidase
• GUS histochemical assay
• PCR analysis
• Cry1ac gene transformation