TAILIEUCHUNG - Lation, characterization and molecular identification of culturable gut bacteria in diamondback moth, Plutella Xylostella (Linnaeus)

Diamondback moth, Plutella xylostella is a major pest of cruciferous crops worldwide and it has developed resistance to almost all synthetic insecticides. It was known to harbour microorganisms which play important role in growth and development of the host. In the present study bacterial strains were isolated from third instar larvae of P. xylostella collected from Sugatur, Kolar District of the state Karnataka. Morphological and Biochemical characterization were done, among them most of the bacterial isolates were gram negative and negative for some biochemical tests. Further, total bacterial genomic DNA was extracted from the bacterial isolates and amplified using PCR with 16S rRNA primers (expected size 1000 bp). Ten different bacterial isolates were isolated and five were identified at genus level such as Serratia marcescens (DBM1 and DBM9), Serratia nematodiphila (DBM2), Serratiasp. (DBM3) and Myroidesodoratus (DBM4). The Serratia spp. is the most predominant bacterial isolate in this region. These studies suggested that a combination of molecular and traditional culturing methods can be effectively used to analyze and determine the diversity of gut microflora. These bacterial strains may play important roles in growth and development of P. xylostella. | Lation, characterization and molecular identification of culturable gut bacteria in diamondback moth, Plutella Xylostella (Linnaeus)

• Sinh học
• Diamondback moth
• Catalase and IMVIC tests
• 16S rRNA
• Serratia spp
• Plutella Xylostella
• Diamond backmoth
• Different insecticidal treatments against diamondback
• Diamondback moth on cabbage
• Nonsignificant with flubendiamide
• Cabbage heads was registered
• Pouzolzia zeylanica
• Leaf extract
• Optimized extraction
• Medical plant
• Biology of diamondback moth
• Plutellae xylostella
• Laboratory condition
• Plutellae Xylostella of cauliflower
• Egg period varies
• Gut bacteria
• Biochemical tests
• Field caught population
• Plutella xylostella L
• Bio efficacy
• Cost effectiveness
• Cabbage at farmers field
• Newer insecticides against diamondback moth
• Geographical localities
• Life fertility
• Intrinsic rate
• Growth rate studies of diamondback moth
• hướng dẫn trồng cây cảnh
• chăn nuôi gia súc
• các loại hình ngư nghiệp
• chăm sóc ngư nghiệp
• hướng dẫn chăn nuôi gia súc
• Bacillus thuringiensis
• Beauveria bassiana
• Bio agents
• Comparative efficacy of bio agents
• BMC Genomics
• Cotesia vestalis
• Diadromus collaris
• Parasitic wasps
• RNA seq
• Alternative splicing
• Sex determination
• Post transcriptional mechanisms