TAILIEUCHUNG - Rapid identification of mycobacterium tuberculosis and non tuberculous mycobacterium isolates from pulmonary and extra pulmonary samples using MGIT320 liquid culture system and mpt64 antigen test

Tuberculosis (TB) is a major public health problem in India and a leading cause of death in adults, especially among the economically productive age group. Historically TB has been associated with significant morbidity and mortality and remains a major global health problem. The present study was initiated to determine the prevalence of Mycobacterium tuberculosis, Non Tuberculous Mycobacterium and its resistance to first line AntiTubercular drug from both pulmonary and extra pulmonary samples A total of 583 properly collected samples (226 pulmonary and 357 extra pulmonary) from patients with clinical/radiological suspicion of Tubercular infection were included in this study. All the samples were screened by Zeihl-Neelsen AFB microscopy, and subjected to liquid culture using Mycobacterium Growth Indicator Tube (MGIT-320). Positive cultures were differentiated into Mycobacterium tuberculosis complex (MTBc) or non-tubercular mycobacterium (NTM) by immunochromatography assay using MPT-64 antigen. Further it was followed by drug susceptibility testing of MTBc isolates thereby identifying multidrug resistant strains. Out of 583 samples, 141 strains were isolated on MGIT-320 (81 pulmonary, 60 Extrapulmonary) and the detection time was 15 days. Mycobacterium complex isolates were 116 and Nontuberculous Mycobacteria were 25. Among Mycobacterium tuberculosis complex isolates 92(56 pulmonary, 36 Extrapulmonary) were sensitive to all the drugs and 24(16 pulmonary, 8 Extrapulmonary) were resistant to one or more drugs. Multiple drug resistant (MDR) isolates were 7(6 pulmonary, 1 Extrapulmonary). MDR-TB is gradually increasing due to improper diagnosis and inadequate treatment. Differentiating mycobacterium as MTBc and NTM supported by sensitivity testing by using liquid culture has proved to be helpful in early decision for chemotherapy in MDR-TB patients. | Rapid identification of mycobacterium tuberculosis and non tuberculous mycobacterium isolates from pulmonary and extra pulmonary samples using MGIT320 liquid culture system and mpt64 antigen test

• Sinh học
• Mycobacterial growth indicator tube
• Mycobacterium tuberculosis complex
• Non tubercular mycobacterium
• MPT64 antigen test
• Multiple drug resistant
• Extrapulmonary tuberculosis
• Nontubercular mycobacterium
• BMC Cancer
• Epithelial mesenchymal transition
• Multiple drug resistance
• Cancer cells
• Aberrant metastatic
• BMC Anesthesiology
• Critical illness
• Subgroup analysis
• Continuous variables
• Allosteric communication
• Closeness centrality
• Betweenness centrality
• Drug resistant bacteria
• Druggable sites
• Triple negative breast cancer
• Cisplatin sensitivity
• Cancer subtypes
• Gene expression
• Cancer genomics
• Drug response
• Functional genomics
• Gyps himalayensis
• MRP4 (Multi Drug Receptor Protein 4)
• ABC (ATP binding cassette) superfamily
• MAP (Multi Antigenic Peptide)
• SPPS (Solid Phase Peptide Synthesis)