TAILIEUCHUNG - Distribution of Oligomannosyl side chains in the cell wall mannan of Pichia Pastoris purified by Benanomicin a

In the previous study, a new method was established to prepare mannan under mild conditions by using antibiotic Benanomicin A. In this study, this method was applied to the isolation of P. pastoris NBRC 0984 mannan which predominantly contains β-1,2- linked mannose residues. As the findings of nuclear magnetic resonance (NMR) analysis of the resultant mannan to examine the distribution state of the side chain, it was found that despite the existence of oligomannosyl side chains corresponding to pentaose, Manα1→2Manβ1→Manβ1→Manα1→2Man, tetraose, Manβ1→Manβ1→Manα1→2Man, and biose, Manα1→2Man, in this molecule, the side chain corresponding to triose, Manβ1→Manα1→2Man, was not detected at all. In our previous study, a relatively large number of biosyl and triosyl side chains were detected in analyzes applying acetolysis to mannans prepared by the Fehling method from the same yeast cells. Such a difference can be explained as that the β-1,2-linkages of some pentaosyl and tetraosyl side chains were cleaved by acetolysis, and triose and biose occurred secondarily in large quantities. In conclusion, the best way to accurately measure the side chain distribution of mannan is to perform NMR analysis on untreated mannan molecules prepared under mild conditions using Benanomicin A. | Distribution of Oligomannosyl side chains in the cell wall mannan of Pichia Pastoris purified by Benanomicin a

• Sinh học
• Pichia pastoris
• Cell wall mannan
• Benanomicin A
• Nuclear Magnetic Resonance
• Pichia Pastoris purified
• Oligomannosyl side chains
• Nghiên cứu cấu trúc nấm men Pichia Pastoris
• Nấm men Pichia Pastoris
• Sàng lọc dòng nấm men Pichia Pastoris
• Tái tổ hợp đa bản sao
• Nhân tố tăng trưởng từ tiểu cầu
• Biotechnology and food sciences
• Recombinant glucoamylase expression
• Native in pichia pastoris
• α mating factor secretion signal in pichia pastoris
• Raw starch degrading glucoamylase
• Aspergillus flavus NSH9
• BMC Biotechnology
• P pastoris
• Co expression
• Immunomodulatory fungal sphingolipid
• Liver injury
• Engineered Pichia pastoris
• Scientific reports
• scientific research
• Structural biochemistry
• expression analysis
• hormone medicine
• Cấu trúc chủng nấm men Pichia pastoris
• Cấu trúc chủng nấm men
• Mini proinsulin dạng tiết
• Môi trường nuôi cấy
• Mã hóa gen
• Human cathepsin C
• Recombinant protein
• Mononuclear cells
• pGEM T cloning vector
• Aspergillus niger
• β mannanase
• Protein disulfide isomerase
• Expression condition optimization
• Pichia pastoris X 33
• Recombinant human paraoxonase
• Saccharomyces cerevisiae alpha factor signal sequence
• High density lipoprotein
• Porcine PID1
• Tissue distribution
• Yeast Pichia pastoris
• Eukaryotic expression
• Nghiên cứu biểu hiện vùng gien preM env
• Vùng gien preM env
• Virút Dengue typ 2
• Tổng hợp vùng gien preM env
• Biểu hiện của Interferon gà tái tổ hợp
• Hoạt tính sinh học của Interferon
• Interferon gà tái tổ hợp
• Hệ thống nấm men Pichia Pastoris
• Bệnh do virus ở gà
• Cải biến mã bộ ba
• Gen mã hóa Asparaginase
• Aspergillus oryzae trong nấm men
• Gen cải biến
• Thermobifida fusca Xyn11A
• C terminal His tag
• Xylanases degrade
• Recombinant protein expression
• AOX1 promoter
• Methanol expression regulator 1
• Synthetic gene circuit
• Pro peptide
• Enzyme activity
• Microbial transglutaminase
• Copper dependent enzymes
• Aspergillus clavatus
• Saccharomyces cerevisiae
• Virus like particles
• Dengue virus
• Envelope glycoprotein
• Envelope domain III
• Neutralizing antibody
• Truncated plasminogen
• Recombinant human microplasminogen
• Human plasmin
• L ornithine
• Recombinant human arginase I
• L arginine
• Myo inositol hexakisphosphate
• Citrobacter amalonaticus phytase
• Endoplasmic reticulum
• Gene cloning
• Expression system
• Penicillium oxalicum
• Protein expression and purification
• Autoimmune diseases
• Thermoalkaliphilic xylanase
• Fermentation parameter optimization
• Fed batch strategy
• High cell density fermentation
• Glycoprotein gene
• CVS rabies virus
• SDS PAGE RTPCR
• Rabies virus glycoprotein gene