TAILIEUCHUNG - Isolation and in silico characterization of disease resistance gene analogues (RGAs) from minor Millets

Minor millets are under-exploited plant species often dubbed as ‘Crops for the future’ as they are best adapted to less fertile area and can survive limited water situations and relatively free from pests and diseases. Degenerate oligo-PCR strategy was employed to isolate resistant gene analogues from minor millets by amplifying samples using NBSLRR gene specific primers. Genomic DNA was isolated from the young leaves of minor millets viz., Foxtail millet (Setaria italica) var. CO 5 and Finger millet (Eleusine corocana) var. CO (Ra) 14 by following CTAB method with minor modifications. PCR amplification of the genomic DNA with degenerate primers, revealed presence of 500bp product in the minor millet samples typical to the size of other reported R-gene analogues. The amplicons TENAIKKM1 sequenced was of size 527 bases and RagiKKM1 was of size 509 bases, the sequences with high similarity to NBS-LRR family are deposited in GenBank, NCBI. In silico characterization of the sequences were done using bioinformatics tools by similarity search, pattern recognition and diversity analysis, which resulted in classifying the genes as those belonging to NBS-LRR class as that of other grass family NBS-LRR kind of proteins. Further, in silico characterization ensured that they belong to Non-TIR family of R genes as evident by the conserved residues in the kinase 1 and kinase2 domains. The diversity among the novel RGAs characterized in this study may lead to mining of R- genes in other under-exploited minor millets. | Isolation and in silico characterization of disease resistance gene analogues (RGAs) from minor Millets

• Sinh học
• Resistant gene
• In silico
• Silico characterization
• Disease resistance gene analogues
• Minor Millets
• Molecular marker
• Resistant gene analogous marker
• Yellow mosaic virus resistant genotypes
• Yellow mosaic virus resistant genotypes in greengram
• Average plant yield
• Chicken meat
• MecA gene
• Pvl gene
• Market samples of chicken meat
• Panton valentine Leukocidin
• Methicillin resistant staphylococcus aureus isolated
• MDR salmonella
• 16S rRNA gene
• 23S rRNA gene
• RNA sequencing method
• Multidrug resistant salmonella serovars isolated
• Varying drug resistant patterns
• MRSA isolates with PVL gene
• MRSA isolates
• Antibiotics except vancomycin
• Polymorphism in Exon 14
• Antiviral resistant Mx Gene
• Indigenous chicken breeds
• Nucleotide polymorphism
• Đa hình nucleotide
• Giống gà bản địa
• Imipenem resistant E coli
• PCR detection
• BlaIMP Gene
• Metallo β Lactamase
• Clinical samples
• Aminoglycoside Acetyl transferase enzyme gene
• Ciprofloxacin resistant isolates
• Enterobacter cloacae
• Klebsiella pneumoniae
• Seventy two urinary
• Virulent gene characterization
• Methicillin sensitive staphylococcus aureus
• Methicillin resistant staphylococcus aureus
• Bovine mastitis
• Staphylococcal enterotoxin genes
• BMC Plant Biology
• Verticillium wilt resistant
• Resistance gene analogues
• RGA gene rich clusters
• Verticillium dahliae response loci
• Staphylococcus aureus
• Coa gene
• Length polymorphism
• PCR restriction fragment
• Staphylococcus aureus based
• Molecular typing of methicillin resistant
• Multidrug resistance
• Antimicrobial resistance
• Gene cassette
• Bacterial species
• Multi antibiotic resistant
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• kiến thức y học
• nghiên cứu y học
• Cf 19 gene
• CSolanum lycopersicum
• Cladosporium fulvum
• Tomato leaf mold
• SLAF seq
• Blast disease
• Pi9 gene
• Foreground selection
• Background selection
• Gene through backcross breeding
• Poultry meat
• Antibiotic Sensitivity
• Poultry house environment
• Detection and molecular characterization
• Methicillin resistant staphylococcus aureus obtained
• Neonatal calf diarrhoea
• Multidrug resistant MDR E coli
• UspA gene
• Carbapenem Resistant
• ESBL producers
• Resazurin microplate assay
• Rapid assay for detection
• Methicillin resistant
• Plant Biology
• Resistant starch
• Starch synthase
• Null mutations
• Health benefits
• BMC Genomics
• Sporisorium scitamineum
• Disease resistance
• Resistance genes
• Immune system of plants
• Breeding pathogen resistant crops
• Klebsiellapneumoni ae
• Blakpc gene
• Blandm 1 gene
• Blaoxa 48gene
• Clinical isolates
• Stem rust
• Spot blotch
• Bipolaris sorokiniana
• Spot blotch of wheat
• Plant genetic engineering
• Cowpea Vigna unguiculata L
• Herbicide tolerance
• N acetyltransferase
• Herbicide tolerance gene
• cáo cáo y học
• Heavy metal resistant bacteria
• Copper bioremoval
• 16S rRNA
• Lysogeny broth
• DNA sequencing