TAILIEUCHUNG - Thiết lập phương pháp lấy mẫu lúa theo tiêu chuẩn ISO/IEC 17025: 2017 phục vụ thử nghiệm xác định locus gen
Tiêu chuẩn ISO/IEC 17025 (International Organization for Standardization/ International Electrotechnical Commission) là một trong những thước đo quan trọng chứng nhận cho phòng thí nghiệm đạt tiêu chuẩn quốc tế. Trong nghiên cứu này, các phương pháp lấy mẫu lúa phục vụ thử nghiệm xác định locus gen mục tiêu đã được thiết lập. | Thiết lập phương pháp lấy mẫu lúa theo tiêu chuẩn ISO/IEC 17025: 2017 phục vụ thử nghiệm xác định locus gen Tạp chí Khoa học Công nghệ Nông nghiệp Việt Nam - Số 11(96)/2018 Predicting, identifying and designing functional marker to identify blast resistance candidate gene (Pit) in Vietnamese rice varieties Nguyen Truong Khoa, Nguyen Thuy Diep, Nguyen Thai Duong, Nguyen Nhu Toan , Phuong Huu Pha, Dang Thi Thanh Ha, Kieu Thi Dung, Tran Thi Thuy, Tran Dang Khanh, Khuat Huu Trung Abstract The rice blast resistance gene Pit, which was identified in an Indonesian indica rice variety, Tjahaja, confers broad- spectrum resistance (Kiyosawa, 1972). Pit gene was isolated and revealed that Pit belongs to the CC-NBS-LRR family of resistance genes (Hayashi and Yoshida, 2009). DNA markers that allow for identification of resistance genes in rice germplasm have a great advantage in resistance breeding because they can assess the existence of the genes without laborious inoculation tests. Functional markers, which are designed from functional polymorphisms within the sequence of genes, are unaffected by nonfunctional allelic variation and make it possible to identify an individual gene. In this study, based on the genome sequence databases of 17 Vietnamese rice varieties, we have predicted and identified the candidate gene Pit that involved in blast resistance ability of 17 local rice varieties. In which, 9 homologous sequences had total nucleotides of less than 3 nucleotides and 8 homologous sequences had total nucleotides of less than 20 nucleotides compared to the reference gene LOC_Os01g05620. Simultaneously, we developed Ins_Pit_17 marker derived from InDels segments of candidate gene Pit in local rice varieties. These results indicate that our functional marker should enhance prediction of Pit function and be applicable in rice breeding programs. Keywords: Candidate gene, Pit, blast resistance, rice, marker Ngày nhận .
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