TAILIEUCHUNG - Understanding colonization and proliferation potential of endophytes and pathogen in planta via plating, polymerase chain reaction, and ergosterol assay

This study aimed to establish the colonization behavior and proliferation potential of three endophytes and one pathogen Ganoderma boninense (Gb) introduced into oil palm ramets (host model). The endophytes selected were Diaporthe phaseolorum (WAA02), Trichoderma asperellum (T2), and Penicillium citrinum (BTF08). Ramets were first inoculated with 100 mL of fungal cells (106 cfu mL 1 ) via soil drenching. For the next 7 days, ramets were sampled and subjected to three different assays to detect and identify fungal colonization, and establish their proliferation potential in planta. Plate assay revealed the presence of endophytes in root, stem and leaf tissues within 7 days after inoculation. Polymerase Chain Reaction (PCR) detected and identified the isolates from the plant tissues. The ergosterol assay (via high-performance liquid chromatography, HPLC) confirmed the presence of endophytes and Gb in planta. The increase in ergosterol levels throughout 49 days was however insignificant, suggesting that proliferation may be absent or may occur very slowly in planta. This study strongly suggests that the selected endophytes could colonize the host upon inoculation, but proliferation occurs at a slower rate, which may subsequently influence the biocontrol expression of endophytes against the pathogen. | Understanding colonization and proliferation potential of endophytes and pathogen in planta via plating, polymerase chain reaction, and ergosterol assay

• Sinh học
• High performance liquid
• Oil palm
• Understanding colonization
• Proliferation potential
• Pathogen in planta via plating
• Polymerase chain reaction
• BMC Chemistry
• Emerging pollutants
• Salting out assisted liquid–liquid extraction
• Potable water
• High performance liquid chromatography–diode array detector
• Journal of Chemistry
• Capsicum annuum L
• Dispersive liquid liquid microextraction
• Prostate cancer
• High performance liquid chromatography
• Solid liquid extraction
• Trace analysis
• Dispersive solvent
• Traditional Chinese medicine
• Chinese medicine oral liquid
• Antimicrobial residues
• Quinolones residues
• Quinolones residues in milk
• Liquid chromatography
• Aqueous two phase system
• Chromatographic determination
• 1 allyl 3 methyl imidazolium bromide
• Agricultural drugs
• Dispersive liquid–liquid microextraction
• Personal care products
• Esters of p hydroxybenzoic acid
• Capillary electrophoresis UV
• Vortex assisted liquid–liquid microextraction
• Green analytical chemistry
• Water samples
• Plasma mass spectrometry
• High–performance liquid chromatography
• Hollow fiber liquid phase microextraction
• High performance liquid chromatography–UV detection
• Experimental design
• Melamine in soil samples
• Fiber liquid phase microextraction
• Chromatography A
• Microfluidic device
• Liquid phase microextraction
• Supported liquid membrane
• Tạp chí khoa học
• Tạp chí y dược
• Y dược quân sự
• Dog plasma
• Smilax glabra
• Multi residue analysis of polar pesticides
• Cleanup procedure combined QuEChERS method
• The analytical method of polar
• Compounds in sediment
• Animal feeds
• Ellmans reagent
• Diode array detection
• Hydrochloric acid
• Tetracycline residues
• RP HPLC
• Solid phase extraction
• Tetracycline residues in milk
• Drug excipient compatibility
• PDA detector
• Analytical wavelength
• Intermediate precision
• Dogs’ plasma
• High performance liquid chromatography spectrometry
• FDA’s regulations
• Linear range
• Extraction efficiency
• Royal jelly
• Trans 10 hydroxy 2 decenoic acid
• Bee feeding
• Different bee feeding
• Ionic liquids ultrasound
• Agastache rugosa
• Reversed phase
• High performance liquid chromatographic
• Ultraviolet detection
• Flickingeria fimbriata
• Acylated C glycosyl flavones
• Multiple stage tandem mass spectrometry
• Quantitative analysis
• Camellia chrysantha
• Golden Camellia
• HPLC method
• Dad detection
• New method development
• Validation for identification
• Quantification of methoxyfenozide insecticide residues
• Methoxyfenozide insecticide residues in grapes
• Insect repellent cream
• Lemongrass oil
• Sắc ký lỏng hiệu năng cao
• Định lượng
• Kem chống côn trùng
• Tinh dầu sả chanh
• Maximum concentration
• Retention time
• Plasma of Pangasianodon hypophthalmus
• Bioavailability of albendazole
• Pesticide residues
• Quantitative analysis of fresh tomatoes
• Rrace of pesticide residues
• Markets in Peshawar
• High performance thin liquid chromatography technique
• Fake protein enhancers
• Animal feed
• Build the determination process of ammelide
• Diode array detector