TAILIEUCHUNG - Regulation of DMT1 on autophagy and apoptosis in osteoblast

Iron overload has recently been associated with the changes in the bone microstructure that occur in osteoporosis. However, the effect of iron overload on osteoblasts is unclear. The purpose of this study was to explore the function of divalent metal transporter 1 (DMT1) in the pathological processes of osteoporosis. | Int. J. Med. Sci. 2017 Vol. 14 275 IVYSPRING ĩvt ơ-rnrt irmrtí clrni t n lí ei-fCMo Tir lT Qriữvtrơc INTERNATIONAL PUBLISHER international journal Oj jVLeaicaiSciences 2017 14 3 275-283. doi Research Paper Regulation of DMT1 on autophagy and apoptosis in osteoblast Fei Liu Wei-Lin Zhang Hong-Zheng Meng Zheng-Yu Cai Mao-Wei Yang Department of Orthopedics the First Hospital of China Medical University Shenyang Liaoning China. El Corresponding author Mao-Wei Yang Department of Orthopedics The First Hospital of China Medical University 155 North Nanjing Street Shenyang Liaoning 110001 China. E-mail ymw69@ FAX 86 24 83283360 Phone 86 24 83283360. Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution CC BY-NC license https licenses by-nc . See http terms for full terms and conditions. Received Accepted Published Abstract Iron overload has recently been associated with the changes in the bone microstructure that occur in osteoporosis. However the effect of iron overload on osteoblasts is unclear. The purpose of this study was to explore the function of divalent metal transporter 1 DMT1 in the pathological processes of osteoporosis. Osteoblast cells were cultured in medium supplemented with different concentrations 0 50 100 200 300 400 500 ụmol L of ferric ammonium citrate FAC as a donor of ferric ions. We used western blotting and immunofluorescence to determine the levels of DMT1 after treatment with FAC. Apoptosis was evaluated by detecting the levels of cleaved caspase 3 BCL2 and BAX with western blotting. Autophagy was evaluated by detecting the levels of LC3 with western blotting and immunofluorescence. Beclin-1 expression was also assessed with western blotting. The autophagy inhibitor 3-methyladenine was used to determine whether autophagy affects the apoptosis induced by FAC. Our .

• Y khoa - Dược
• Divalent metal transporter 1
• Bone microstructure
• Ferric ammonium citrate
• Dimethyl sulfoxide
• Diphenyltetrazolium bromide
• Type 2 diabetes
• Micro CT
• Divalent metal ion transporter 1
• Bone mineral density
• Insulin sensitivity index
• BMC Chemistry
• Anti cancer
• Parenteral delivery
• Response surface methodology
• High energy emulsification method