TAILIEUCHUNG - Novel angiotensin-converting enzyme inhibitory peptides from caseins and whey proteins of goat milk

Angiotensin-converting enzyme (ACE) plays a central role in blood pressure regulation by producing the vasoconstrictor angiotensin II. The inhibition of ACE with natural inhibitors, as alternatives to avoid the side effect of synthetic drugs, is a major target in the prevention of hypertension. In this study, we examined the separated caseins and whey proteins of goat milk for the presence of ACE inhibitory peptides. Digestion of isolated whey proteins and caseins of goat milk by gastric pepsin generated soluble hydrolysates exhibiting significant inhibition of ACE compared to weak inhibition by undigested proteins. The hydrolysates were fractionated by size exclusion chromatography, Sephacryl S-100 column, into four fractions (F1–F4). The late-eluting fraction (F4) of either whey or caseins exhibited greater ACE inhibition. Peptides in both F4 fractions, isolated by RP-HPLC, exhibited variable ACE inhibitory activities with the hydrophobic peptide peaks being the most potent ACE inhibitors. MALDI-TOF MS/MS resulted in identification of three potent ACE inhibitory peptides: PEQSLACQCL from b-lactoglobulin (residues 113–122), QSLVYPFTGPI from b-casein (residues 56–66), and ARHPHPHLSFM from j-casein (residues 96–106). The peptides from whey and caseins exert significant ACE inhibitory activities comparable to that of captopril, an antihypertensive drug, exhibiting IC50 values of lM and lM, respectively. The results introduce, for the first time, new potent ACE-inhibitory peptides that can be released by gastric pepsin of goat milk whey and caseins and thus may pave the way for their candidacy as anti-hypertensive bioactive peptides and prevention of associated disorders. | Novel angiotensin-converting enzyme inhibitory peptides from caseins and whey proteins of goat milk

• Sinh học
• Goat milk
• Bioactive peptides
• Angiotensin I converting enzyme
• Anti hypertension
• Proteins of goat milk
• Human nutrition and health
• Goat milk in human nutrition
• Composition of goat milk
• Milk carbohydrat
• Red pumpkin
• Red pumpkin pulp
• Chemical analysis of goat milk Basundi
• Studies on chemical analysis
• Goat milk whey
• Studies on sensory analysis
• Goat milk whey based herbal beverage
• Colour and appearance
• Overall acceptability
• Method of preparation
• Mozzarella cheese
• Yield of goat milk Mozzarella cheese
• Preparation on yield
• Method of preparation on yield
• Minerals of Jamunapari
• Field and farm
• Farm rearing condition
• Jamunapari goat milk minerals
• Goat and Sheep milk
• αlactoalbumins and β lactoglobulin
• Sheep milk
• Overview of functionality of goat
• Natural functional milks
• Cereal bran
• Probiotic bifidobacterium longum BB536
• Viable existence
• Fermented goat milk supplemented
• Different cereal bran
• Goat’s milk
• Goat’s milk yoghurt
• Frozen storage
• Physical properties
• Sensory acceptability
• In vitro assessment
• Antibiotic resistance pattern
• Lactobacillus strains isolated
• Growth of bifidobacterium longum BB536
• Cereal bran on growth
• Fermentation of goat milk
• Surti goat
• Milk yield in singlet
• Udder morphology relationship
• Bearing Surti goat
• Production performance
• Buffalo milk
• Cow milk
• Dairy products collected
• Physico chemical Properties of milk
• Sensory analysis
• Goat milk basundi
• Panel of judges
• Staphylococcus aureus
• Antibiotic resistance
• mecA gene
• Detection of the mecA gene
• Fat globule size
• Lactation stage
• Attappady black
• Indigenous goat breeds in Kerala
• Milk fat globule size
• Pantja goat
• Effect of lactation stage
• Different lactation stages on minerals
• Different lactation stages
• High rate of prolificacy
• Acid degree value
• Free fatty acid
• Milk quality
• Somatic cell counts
• Subclinical mastitis
• Molai goat
• Composition and nutritive value
• Nutritive value of milk
• Molai breed doe
• Tamil Nadu
• Milk production
• Mineral mixture supplement
• Pathway for livelihood security
• Farm women
• Goat rearing
• Bacteriological study
• Serodetection and bacteriological
• Brucellosis in goats
• Milk samples and vaginal
• Unorganized goat farms