TAILIEUCHUNG - In vitro expression of enolase from Streptococcus suis serotype 2 and it’s antigenicity

Streptococcus suis serotype 2 (SS2) is one of the most important pathogens in the porcine industry and an important zoonotic agent. The absence of suitable vaccine or virulence markers makes SS2 infections more difficult to control. An immunoproteomics approach is used for identifying antigenic proteins in SS2 recognized enolase, which may represent strainspecific antigenic proteins and potential protective antigens. This study aims to clone, express enolase gene from SS2 and use western blotting to evaluate the antigenicity. Enolase gene from the SS2 strain was amplified with specific primers. The obtained PCR product was inserted into an expression vector, pGEX4T1. The recombinant vector was then transformed into BL21 cells for protein expression. Subsequently, the immunological activity of the recombinant enolase was tested by western blotting with human sera in the convalescent phase. The glutathione S-transferase (GST)-tag fusion enolase was purified by glutathione sepharose affinity chromatography for further studies. The SS2 recombinant enolase was successfully expressed in E. coli. The western blotting analysis demonstrated that enolase has an antigenic property, which is recognized by patients naturally infected with SS2. | In vitro expression of enolase from Streptococcus suis serotype 2 and it’s antigenicity

• Sinh học
• In vitro expression
• Enolase from Streptococcus suis serotype 2
• Streptococcus suis serotype 2
• Protein expression
• Represent strainspecific antigenic proteins
• Heat stress
• Gene expression
• In vitro matured bovine oocytes
• Pro and anti apoptotic genes
• Expression profile
• Amelioration by antioxidants
• Interleukin 11
• Interleukin 11 receptor a
• Leukemia inhibitory factor
• DNA sequence
• In vitro fertilization
• BY 2 cells
• Agrobacterium tumefaciens mediated transformation
• In vitro culture
• GUS transient expression
• Chemical and biological
• BMC Molecular and Cell Biology
• Fallopian tube secretory epithelial cells
• Gene expression microarray
• Three dimensional in vitro models
• Tissue microenvironment
• Ovarian cancer
• Primary porcine proximal tubular cells
• Transporter expression
• Cellular uptake
• In vitro model
• Porcine Sox6
• Expression and purification
• Escherichia coli
• In vitro assay
• E
• coli Rosetta
• BMC Plant Biology
• Uptake kinetics
• Phosphate transporter
• In vitro micropropagated
• BMC Cancer
• Gastric cancers
• Cancer stem cells
• Expression signature
• Biomarkers underlying CD133
• Circadian gene
• Cell cycle
• Expression profiling
• Regulatory systems
• Spermatogonial stem cells
• Characterization of Spermatogonial stem cells
• Pre pubertal mice
• Groups showed expression
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