TAILIEUCHUNG - Alcohol dehydrogenase of Candida albicans triggers differentiation of THP-1 cells into macrophages

Candida albicans proteins located on the cell wall and in the cytoplasm have gained great attention because they are not only involved in cellular metabolism and the maintenance of integrity but also interact with host immune systems. Previous research has reported that enolase from C. albicans exhibits high immunogenicity and effectively protects mice against disseminated candidiasis. In this study, alcohol dehydrogenase (ADH) of C. albicans was cloned and purified for the first time, and this study focused on evaluating its effects on the differentiation of the human monocytic cell line THP-1. The morphological features of THP-1 cells exposed to ADH were similar to those of phorbol-12-myristate acetatedifferentiated (PMA-differentiated) macrophages. Functionally, ADH enhanced the adhesion, phagocytosis, and killing capacities of THP-1 cells. A flow cytometric assay demonstrated that ADH-induced THP-1 cells significantly increased CD86 and CD11b expression. The production of IL-1b, IL-6, and TNF-a by cells increased in the presence of ADH. As expected, after pretreatment with a MEK inhibitor (U0126), ADHinduced THP-1 cells exhibited unaltered morphological features, eliminated ERK1/2 phosphorylation, prevented CD86/CD11b upregulation and inhibited pro-inflammatory cytokine increase. Collectively, these results suggest that ADH enables THP-1 cells to differentiate into macrophages via the ERK pathway, and it may play an important role in the immune response against fungal invasion. | Alcohol dehydrogenase of Candida albicans triggers differentiation of THP-1 cells into macrophages

• Sinh học
• Alcohol dehydrogenase
• Moonlighting proteins
• Candida albicans
• Macrophage differentiation
• ERK pathway
• Tomato alcohol dehydrogenase
• Dalziel coefficients
• Enzyme kinetics
• Kinetic characterization
• Report medicine
• traditional medicine
• scientific reports
• immune system
• Antimicrobial
• biosynthesis
• Plant Biology
• Cinnamyl alcohol dehydrogenase
• Cell wall
• Flax fibre
• Linum usitatissimum
• Fusarium oxysporum
• Lạm dụng rượu
• Alcohol dehydrogenase 1B
• Mã hóa enzyme chuyển hóa rượu
• Đa hình đoạn gen ADH1B
• PCR khuếch đại vùng gen
• Journal of Chemistry
• Polypyrrole–polyvinylsulfonate (PPy PVS)
• Nicotinamide adenine dinucleotide (NAD+)
• Meldola’s blue (MB)
• BMC Cancer
• Promoter methylation
• Colorectal cancer
• Aberrant methylation
• Iron containing
• Alcohol dehydrogenase iron containing 1
• Quantitative methylation specific polymerase
• Alcohol induced hypermethylation
• Oxylipin pathway
• Hydroperoxide lyase
• Tomato volatiles
• Alcohol compounds
• Adh null mutations
• Drosophila melanogaster
• Partial dominance
• ADH polymorphism
• Alcohol drinking
• Case control studies
• Esophageal cancer
• Aldehyde dehydrogenase 2
• Alcohol consumption
• Hepatocellular carcinoma
• Overall survival
• Recurrence free survival
• Expression level
• Prognostic value
• BMC Genomics
• Alcohol dehydrogenases
• Cold tolerance
• ROS homeostasis
• Genome wide analysis
• scientific research
• biochemistry
• intermediate cells
• cell proliferation
• medical analysis
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Báo cáo sinh học hay
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• luận văn
• nghiên cứu khoa học
• luận văn sinh học
• chu trình sinh tổng hợp lignin
• gen mã hóa
• sinh vật nhân chuẩn
• biochemical studies
• environmental medicine
• Crystal structure
• oxidation
• chemical reaction
• tumor cells
• breast cancer
• biological activities
• medical knowledge
• cytoplasm
• analysis of cytoplasmic