TAILIEUCHUNG - Preclinical studies of toxicity and safety of the AS-48 bacteriocin

The in vitro antimicrobial potency of the bacteriocin AS-48 is well documented, but its clinical application requires investigation, as its toxicity could be different in in vitro (haemolytic and antibacterial activity in blood and cytotoxicity towards normal human cell lines) and in vivo (. mice and zebrafish embryos) models. Overall, the results obtained are promising. They reveal the negligible propensity of AS-48 to cause cell death or impede cell growth at therapeutic concentrations (up to 27 lM) and support the suitability of this peptide as a potential therapeutic agent against several microbial infections, due to its selectivity and potency at low concentrations (in the range of – lM). In addition, AS-48 exhibits low haemolytic activity in whole blood and does not induce nitrite accumulation in non-stimulated RAW macrophages, indicating a lack of pro-inflammatory effects. The unexpected heightened sensitivity of zebrafish embryos to AS-48 could be due to the low differentiation state of these cells. The low cytotoxicity of AS-48, the absence of lymphocyte proliferation in vivo after skin sensitization in mice, and the lack of toxicity in a murine model support the consideration of the broad spectrum antimicrobial peptide AS-48 as a promising therapeutic agent for the control of a vast array of microbial infections, in particular, those involved in skin and soft tissue diseases. | Preclinical studies of toxicity and safety of the AS-48 bacteriocin

• Y khoa - Dược
• Antimicrobial peptides
• Topical delivery
• Zebrafish model
• Mouse model
• Safety of the AS 48 bacteriocin
• Fish disease
• Therapeutic alternative
• Hyalophora cecropia
• BMC Genetics
• Drosophila melanogaster
• Amyloid β peptides
• Transcription factor FOXO
• Peroxiredoxin 5
• Biochemistry
• molecular Biology
• biological cells
• innate immunity
• cytoplasm
• BMC Genomics
• Feature weighting
• Molecular descriptors
• Multi objective evolutionary algorithm
• Peptide representation
• Antimicrobial resistance
• Antimicrobial peptide
• Deep learning
• Feature fusion
• Organisms against microbes
• scientific reports
• thesis report
• medical report
• medical knowledge
• medical research
• analysis of cytoplasmic
• breast cancer
• medicine
• chemical reaction
• Crystal structure
• medical analysis
• biochemical studies
• BMC Plant Biology
• Plant immunity
• Physcomitrella patens
• Cryptic peptides
• Functionally active proteins
• Intercellular fluid
• Cysteine rich peptides
• Heterologous expression
• Transgenic plants
• Vacuum infiltration
• Data independent acquisition
• Trình bày báo cáo
• tài liệu báo cáo khoa học
• nghiên cứu y học
• báo cáo y học
• kiến thức y học
• Report medicine
• traditional medicine
• Cell penetrating peptides
• Antimicrobial peptide LL 37
• RNA interference
• Scanning tunneling electron microscopy
• Protein bodies
• Pathogen resistance
• Fusarium verticillioides
• Dickeya dadantii
• Oryza sativa
• BMC Musculoskeletal Disorders
• Multiple trauma
• Human beta defensin
• Real time PCR
• Parallel identification
• Signal peptide region
• Journal of Zoology
• Foam nest
• Mass spectrometry
• Protein profiling
• Biosynthesis gene clusters
• Genome mining
• Ribosomally synthesized post translationally modified peptides
• Non ribosomal peptides
• immune system
• Antimicrobial
• biosynthesis
• scientific research
• Equus asinus
• genetics
• Structural biochemistry
• Structural Biology
• tumor cells