TAILIEUCHUNG - Analysis of structure and differential expression of Pseudomonas syringae 5-like (RPS5-like) genes in pathogen-infected Vitis flexuosa

Nucleotide binding site-leucine-rich repeat (NBS-LRR) genes are the largest gene resistance family in plants. Resistance to Pseudomonas syringae 5 (RPS5) is a member of the NBS-LRR family. RPS5 and its homologs are important disease resistance genes in plants including Arabidopsis. | Turkish Journal of Biology Research Article Turk J Biol (2015) 39: 775-789 © TÜBİTAK doi: Analysis of structure and differential expression of Pseudomonas syringae 5-like (RPS5-like) genes in pathogen-infected Vitis flexuosa Md. Zaherul ISLAM, Soon Young AHN, Hae Keun YUN* Department of Horticulture and Life Science, Yeungnam University, Gyeongsan, Republic of Korea Received: Accepted/Published Online: Printed: Abstract: Nucleotide binding site-leucine-rich repeat (NBS-LRR) genes are the largest gene resistance family in plants. Resistance to Pseudomonas syringae 5 (RPS5) is a member of the NBS-LRR family. RPS5 and its homologs are important disease resistance genes in plants including Arabidopsis. This study identified nine loci of RPS5-like genes in Vitis flexuosa that showed differential expression from transcriptome analysis by next generation sequencing (NGS) of V. flexuosa infected with Elsinoe ampelina based on structural analyses: VfRPS5-like1059 (Vitis flexuosa resistance to Pseudomonas syringae 5-like 1059), VfRPS5-like1833, VfRPS5-like4135, VfRPS5-like4833, VfRPS5-like6172, VfRPS5-like13564, VfRPS5-like20585, VfRPS5-like55532, and VfRPS5-like62178. These genes, 2236–4762 bp in length, exhibited the structure of a coiled coil-nucleotide binding site-leucine-rich repeat (CC-NBS-LRR) resistance gene. The predicted amino acid sequences of all nine genes contained typical NBS domains. Chromosomal localization revealed that the nine genes were located in six chromosomes of the V. flexuosa pseudo-genome. Of the nine loci, only VfRPS5-like55532 showed upregulated expression against all tested pathogens at all tested time points, except 24 h postinoculation (hpi) for E. ampelina. All tested genes also showed differential expression against major grapevine fungal and bacterial pathogens. The results presented herein will also serve as a useful reference dataset for .

• Sinh học
• R gene
• Resistance to Pseudomonas syringae 5
• Elsinoe ampelina
• Rhizobium vitis
• Botrytis cinerea
• Colletotrichum gloesporioides
• Colletotrichum acutatum
• Khả năng ức chế của Nanochitosan
• Hiệu lực ức chế
• Gâu hại cà chua
• Cà chua sau thu hoạch
• Calcium alginate
• Antifungal activity
• Cassia oil
• Essential oil
• Czepek’s Dox agar
• Peptone salt agar
• Oat meal agars
• Various carbon sources
• Tạp chí Bảo vệ thực vật
• Nấm Colletotrichum acutatum
• Bệnh thán thư
• Sâm Ngọc Linh (Panax vietnamensis Ha et Grushv )
• Tách chiết DNA
• Triệu chứng bệnh thán thư Sâm Ngọc Linh
• Capsicum frutescens
• Colletotrichum truncatum
• Đối kháng nấm bệnh thán thư
• Trichoderma koningii
• Nấm bệnh thán thư
• Bệnh thán thư trên cây ớt
• Phương pháp phân lập Tricho derma
• Phương pháp lây bệnh nhân tạo
• kỹ năng nuôi trồng
• kỹ thuật trồng trọt
• cách trồng cây
• Kỹ thuật gieo cấy lúa xuân
• mẹo trồng trọt
• Sản xuất thủy canh dâu tây
• Chất lượng dâu tây trồng
• Năng suất dâu tây
• Bệnh thối mốc xám