TAILIEUCHUNG - Identification and evolutionary genomics of novel LTR retrotransposons in Brassica

Retrotransposons (REs) are the most abundant and diverse elements identified from eukaryotic genomes. Using computational and molecular methods, 262 intact LTR retrotransposons were identified from Brassica genomes by dot plot analysis and data mining. | Turkish Journal of Biology Turk J Biol (2015) 39: 740-757 © TÜBİTAK doi: Research Article Identification and evolutionary genomics of novel LTR retrotransposons in Brassica 1, 2 1 Faisal NOUROZ *, Shumaila NOREEN , John Seymour HESLOP-HARRISON Molecular Genetics Laboratory, Department of Biology, University of Leicester, UK 2 Molecular Genetics Laboratory, Department of Genetics, University of Leicester, UK 1 Received: Accepted/Published Online: Printed: Abstract: Retrotransposons (REs) are the most abundant and diverse elements identified from eukaryotic genomes. Using computational and molecular methods, 262 intact LTR retrotransposons were identified from Brassica genomes by dot plot analysis and data mining. The Copia superfamily was dominant (206 elements) over Gypsy (56), with estimated intact copies of ~1596 Copia and 540 Gypsy and ~7540 Copia and 780 Gypsy from Brassica rapa and Brassica oleracea whole genomes, respectively. Canonical Copia and Gypsy gag-pol polyprotein organizations were observed in most elements with a few displaying 1–3 additional or internally deleted domains. The PBS and PPT motifs were identified with tRNA complementary to tRNAMet or, rarely, other tRNA types. PCR amplification of RT regions revealed their abundance and distribution among A-, B-, and C-genome Brassicas indicating a common ancestor. The evolutionary relationship of Brassica REs resolved them into superfamily-specific (Copia/Gypsy) lineages. The phylogenetic analysis of 130 Brassica Copia clustered them into 2 clades and 10 sub-clades of 18 families; Gypsy elements clustered into 2 clades. The results enabled identification and understanding of the structure and nature of full-length REs and their derivatives in Brassica. The markers derived here will be useful for examining chromosome and genome evolution in Brassica. Key words: LTR retrotransposons, Brassica, Copia,

• Sinh học
• LTR retrotransposons
• Evolutionary relationship
• RTAP markers
• Mobile genetic elements
• Reverse transcriptase
• BMC Genomics
• LTR retrotransposons
• LTR retrotransposons dynamics Comparative genomic
• Long Terminal Repeat
• BMC Plant Biology
• Genomic distribution
• Comparative analysis
• Eucalyptus genomes
• Long terminal repeat retrotransposon
• Lamellibrachia luymesi
• Previously recognized
• G4 motifs
• Quadruplex DNA
• Transposable elements
• Maize LTR retrotransposons
• Circular dichroism
• NMM ligand
• Genome Biology
• Circular RNA
• Satellite RNA
• Catalytic RNAs
• Transcriptomic retrozyme sequences
• biology
• biological research
• applied biology
• modern biology
• biological engineering research
• metazoan systems
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Active transposon families
• Genome diversity
• Miniature inverted–repeat transposable elements
• Chromosome mapping
• Molecular cytogenetics
• Predominant contributors
• Bioinformatics analysis
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Long terminal repeat retrotransposons
• DNA methylation status
• Random amplified polymorphic DNA
• Coupled restriction enzyme digestion random amplification
• Plant Biology
• Genome structure and evolution
• Plant retrotransposon dynamics
• Retrotransposon expression
• Retrotransposon insertion time
• Ficus carica L
• Wheat genome
• Genome evolution