TAILIEUCHUNG - Mating type groups of ascochyta rabiei (Teleomorph: Didymella rabiei), the causal agent of chickpea blight in Central Anatolia

The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. | Turk J Agric For 31 (2007) 41-46 © TÜB‹TAK Mating Type Groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the Causal Agent of Chickpea Blight in Central Anatolia Harun BAYRAKTAR*, F. Sara DOLAR, Salih MADEN Department of Plant Protection, Faculty of Agriculture, University of Ankara, 06110, Ankara - TURKEY Received: Abstract: The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production were observed on chickpea stem pieces that were incubated in 8 ± 1 °C and 85% relative humidity. Both mating types were found in all the provinces, except for Kayseri and Sivas. The majority of the isolates belonged to Mat 1-1 () and the others () to MAT 1-2. Additionally, the mating types of A. rabiei were differentiated from each other using the multiplex PCR method with the primers Com1, Tail 5, and SP21. MAT 1-1 isolates were amplified to approximately a 460 bp fragment and MAT 1-2 isolates to a 700 bp fragment, in accordance with the discrimination of mating type groups with conventional methods. Key Words: Chickpea, Ascochyta rabiei, multiplex PCR, mating type Nohutlarda Yan›kl›k Etmeni Ascochyta rabiei’nin (Teleomorph: Didymella rabiei) Orta Anadolu Bölgesindeki Mating Type Gruplar› Özet: Orta Anadolu bölgesindeki 6 farkl› nohut ekim alan›ndan toplanan 45 Ascochyta rabiei (teleomorph: Didymella rabiei) izolat›n›n mating type gruplar›n›n da¤›l›m› laboratuar koflullar›nda araflt›r›lm›flt›r. Nohut gövde parçalar› üzerinde pseudothecium geliflimi ve ascospor üretimi 8 ± 1 °C s›cakl›k ve % 85 nispi nem içeren koflullarda 5-6 haftal›k inkübasyon periyodundan sonra gözlenmifltir. Kayseri ve Sivas illeri hariç di¤er tüm illerde her iki mating type grubu da .

• Nông nghiệp
• Chickpea blight
• Ascochyta rabiei
• Multiplex PCR method
• Mating type
• Genomic DNA
• Chickpea Cicer arietinum L
• Chickpea cultivars
• Ascochyta blight of chickpea
• Yield of chickpea in Turkey
• Ascochyta blight resistance in chickpea
• Ascochyta blight
• Quantitative trait locus
• Recombinant inbred line
• Molecular markers
• Cultivated chickpea Cicer arietinum L
• Cicer arietinum
• Seed yield
• Yield criteria
• Inoculation and resistance
• Biochemical parameters
• Ascochyta blight infected moderately resistant
• Susceptible chickpea genotypes
• Inoculated leaves
• Journal of Agriculture and Forestry
• Production economically
• Chickpea production
• Simple genomic DNA extraction
• Efficient and universal method
• The extraction of genomic DNA
• Genomic DNA from bacteria
• Microalgae suitable
• PCR based applications
• DNA tái tổ hợp
• công nghệ gen
• kỹ thuật di truyền
• kỹ thuật gen
• khoa học tự nhiên
• sinh học
• Pigeon pea
• Genomic DNA extraction
• Genomic DNA extraction from pigeon pea
• Rapid procedure
• Showed highly pure DNA
• Oryza sativa
• Solanum lycopersicum
• DNA extraction
• DNA purity
• Thư viện DNA bộ gene
• Bài giảng Thư viện DNA bộ gen
• Thư viện DNA
• Sàng lọc dòng DNA
• DNA genomic library
• Thử miễn dịch
• Genomic DNA isolation
• Guanidinium isothiocyanate
• DNA isolation methods
• Microbiology laboratories
• Staphylococcus aureus
• Rapid and efficient procedure
• Trichoderma spp
• DNA with high yield
• PCR purposes
• BMC Bioinformatics
• Comparative genomics
• Alignment free
• Genomic signature
• Information distance
• Additive DNA signature
• Composite DNA signature
• Assembled DNA signature
• BMC Genetics
• DNA methylation
• 5 azacytidine
• Genomic binding
• Remodel chromatin
• DNA demethylating reagent
• BMC Genomics
• DNA epigenome
• DNA N6 methyladenosine
• DNA N4 methylcytosine
• Biological functions
• báo cáo thực tập
• luận văn tốt nghiệp
• thư viện cDNA
• thư viện genomic DNA
• Lai insitu
• hoạt tính enzym
• Journal of Botany
• Aluminum stress
• iPBS profile
• Genomic instability
• Reactive oxygen species
• Primary osteoporosis
• Bone remodeling
• Osteoporotic fracture bone tissues
• DNA sequencing
• Molecular cytogenetic identification
• Wheat–Thinopyrum intermedium introgression line
• Total genomic DNA
• Wheatgrass chromosomes
• ABD genomic DNA
• SEQUENCING REACTION
• STRAND SEPARATION
• PRIMER ANNEALING
• PRIMER EXTENSION
• DNA PREPARATION
• Phenotypic plasticity
• Genomic data
• Sociality through DNA Methylation
• Epigenetic control of sociality
• Ancient DNA
• DNA contamination removal
• Competitive mapping
• Cost effective sequencing
• High throughput sequencing
• Genome Biology
• Genomic imprinting
• Oocyte specific epigenetic
• Epigenetic phenomenon
• Bimodal methylation
• Cinnamomum verum Presl
• Cinnamomum sp
• Cinnamomum species
• Commercial DNA isolation kits
• Yield DNA
• Species concept
• Genome sequences
• DNA DNA hybridization
• Prokaryotic species
• Preimplantation embryos
• Epigenetic reprogramming
• DNA methyltransferases
• 6mA modification
• DNA variation
• RNA variation
• Transmitted variation
• Genomic feature analysis
• Information theory
• Mutual Information
• Gene ranking
• Methylation analysis
• Clinico genomic model
• High dimensional data
• Logistic regression