TAILIEUCHUNG - Effects of Turkish propolis extract on secretion of polymorphonuclear elastase following respiratory burst

Propolis is a natural, resinous, hive product with various bioactive properties. Polymorphonuclear leukocytes (PMNLs) use both oxygen-dependent (respiratory burst) and oxygen-independent mechanisms (releasing proteolytic enzymes such as elastase) to destroy infectious agents. | Turkish Journal of Biology Research Article Turk J Biol (2015) 39: 194-201 © TÜBİTAK doi: Effects of Turkish propolis extract on secretion of polymorphonuclear elastase following respiratory burst 1 1 1, 2 1 Yaşam BARLAK , Orhan DEĞER *, Meltem UÇAR , Tuğba Nigar ÇAKIROĞLU Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey 2 School of Health Sciences, European University of Lefke, Lefke, Turkish Republic of Northern Cyprus Received: Accepted: Published Online: Printed: Abstract: Propolis is a natural, resinous, hive product with various bioactive properties. Polymorphonuclear leukocytes (PMNLs) use both oxygen-dependent (respiratory burst) and oxygen-independent mechanisms (releasing proteolytic enzymes such as elastase) to destroy infectious agents. The in vitro effects of dimethyl sulfoxide (DMSO) extract of Turkish propolis on the secretion of PMN elastase from PMNLs following respiratory burst was studied. Phorbol myristate acetate was used to stimulate respiratory burst. The Flow Cytometric CellProbe Oxidative Burst Test was used to determinate if respiratory burst occurred or not and what its severity was after a 4 h incubation with propolis extracts (0 mg/mL, mg/mL, mg/mL, mg/mL, and 5 mg/mL). PMN elastase activity following respiratory burst was determined by Flow Cytometric CellProbe Elastase Test. At the 5 mg/mL concentration, the percentages of fluorescence positivities of DCFH, PMA Oxidative Burst Test were observed as 28 ± and 27 ± for untreated and PMA-stimulated PMNLs, respectively. At the concentration of 5 mg/mL, percentage of fluorescence positivities of RGES-Elastase Test were observed as ± and ± for untreated and PMA-stimulated PMNLs, respectively. It was concluded that DMSO extracts of Turkish propolis may exhibit .

• Sinh học
• Respiratory burst
• Polymorphonuclear elastase
• Polymorphonuclear leukocyte
• Oxygen dependent
• Oxygen independent mechanisms
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• β hydroxy β methylbutyrate
• Healthy immune system plays
• Chemotactic activity
• Murraya paniculata
• DPPH radical scavenging assay
• Lipoxygenase inhibition assay
• Prolyl endopeptidase inhibition assay
• Respiratory burst inhibitory assay
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• Plant NADPH oxidase
• EF hands
• Interacting partners
• Phosphorylation sites
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• Upland cotton
• Expression patterns
• Gene family
• Phaseolus vulgaris
• NADPH oxidases
• Respiratory burst oxidase homologs
• Arbuscular mycorrhizal
• Innate immunity
• Immune system
• Homeothermic mice
• Phagocyte activity
• Macrobrachium rosenbergii
• White muscle disease
• Growth and survival
• Disease resistance
• Phenoloxidase activity