TAILIEUCHUNG - A simple guanidinium isothiocyanate method for bacterial genomic DNA isolation
A high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study, we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. | Turkish Journal of Biology Turk J Biol (2014) 38: 125-129 © TÜBİTAK doi: Research Article A simple guanidinium isothiocyanate method for bacterial genomic DNA isolation 1,2, 1 2 1,3 4,5 Erkan MOZİOĞLU *, Müslüm AKGÖZ , Candan TAMERLER , Zühtü Tanıl KOCAGÖZ Molecular Biology, Biotechnology, and Genetics Research Center, İstanbul Technical University, İstanbul, Turkey 2 Bioanalysis Laboratory, TÜBİTAK National Metrology Institute (UME), Kocaeli, Turkey 3 Mechanical Engineering Department, University of Kansas, Lawrence, Kansas, USA 4 Department of Microbiology and Clinical Microbiology, Acıbadem University, İstanbul, Turkey 5 Trends in Innovative Biotechnology Organization, İstanbul, Turkey Received: Accepted: Published Online: Printed: Abstract: A high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study, we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. The results show that the modified guanidinium isothiocyanate method developed in our laboratory is simple, fast, and inexpensive and yields DNA whose quality and quantity are similar to that of 2 commercial extraction kits but far better than 2 conventional DNA extraction methods used for comparison. Key words: Bacteria, genomic DNA isolation, guanidinium isothiocyanate 1. Introduction In environmental (Bürgmann et al., 2001), clinical (Kocagöz et al., 1993), and food (Elizaquível and Aznar, 2008) microbiology laboratories, high-quality DNA is required for identification of microorganisms in samples. For this purpose, several methods have been developed and are in use. These methods can be classified into 2 major groups: inhouse extraction methods and methods
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