TAILIEUCHUNG - In vitro propagation of some new banana types (Musa spp.)

Three newly selected superior banana types were used to study the effects of different cytokinins and auxins on shoot multiplication and rooting. Benzylaminopurine [(BAP) (5, 10, 20 and 30 µM)] and thidiazuron [(TDZ) (, 1, 2 and 3 µM)] were tested alone and with 1 µM indoleacetic acid (IAA) for the propagation stage. | Turk J Agric For 28 (2004) 355-361 © TÜB‹TAK In Vitro Propagation of Some New Banana Types (Musa spp.) Hamide GÜBBÜK*, Mustafa PEKMEZC‹ Department of Horticulture, Faculty of Agriculture, Akdeniz University 07059 Antalya - TURKEY Received: Abstract: Three newly selected superior banana types were used to study the effects of different cytokinins and auxins on shoot multiplication and rooting. Benzylaminopurine [(BAP) (5, 10, 20 and 30 µM)] and thidiazuron [(TDZ) (, 1, 2 and 3 µM)] were tested alone and with 1 µM indoleacetic acid (IAA) for the propagation stage. We compared basal Murashige and Skoog (MS) -1 medium, active charcoal (5 g l ), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) for rooting. Shoot proliferation and elongation were significantly greater with TDZ than with BAP all 3 types. Furthermore, each cytokinin with IAA increased shoot proliferation and elongation more than their use alone. BAP below 20 µM or TDZ below 1 µM did not increase shoot proliferation, and BAP over 20 µM and TDZ over 2 µM suppressed shoot elongation. Charcoal alone was better for rooting than auxin treatments or MS medium alone. In conclusion, supplementation of 2 µM TDZ, and 1 µM IAA or 20 µM BAP and 1 µM IAA on MS medium, followed 5 g l-1 charcoal at the rooting stage were the best combinations for the in vitro propagation of banana types. Key Words: Banana, Musa spp., tissue culture, thidiazuron, active charcoal Baz› Yeni Muz Tiplerinin (Musa spp.) Doku Kültürü ile Ço¤alt›lmas› Özet: Bu çal›flmada deneme materyali olarak, yeni selekte edilmifl 3 superior muz tipi kullan›lm›fl ve muz tiplerinin ço¤alt›lmas› ve köklendirilmesi üzerine farkl› sitokinin ve oksin tiplerinin etkileri araflt›r›lm›flt›r. Bu amaçla, ço¤altma aflamas›nda de¤iflik benzylaminopurine [(BAP) (5, 10, 20 and 30 µM)] ve thidiazuron [(TDZ) (, 1, 2 and 3 µM)] konsantrasyonlar›n›n ba¤›ms›z ve 1 µM indoleacetic asit (IAA) ile olan kombinasyonlar› test edilmifltir. Ayr›ca .

• Nông nghiệp
• Banana types Musa spp
• Musa spp
• Tissue culture
• Active charcoal
• Indoleacetic acid
• Plant growth regulators
• In vivo
• Lipid peroxidation
• Kidney tissue
• PGR treated
• Serum enzymes
• Indolebutiric acid
• Aspartate aminotransferase