TAILIEUCHUNG - Isolation and characterization of native Bacillus thuringiensis isolates from Syrian soil and testing of their insecticidal activities against some insect pests

This study constitutes the first isolation and characterization of local B. thuringiensis isolates in Syria. Some of these isolates exhibit toxic potential, therefore could be adopted for future applications to control some important insect pests. | H. AMMOUNEH, M. HARBA, E. IDRIS, H. MAKEE Research Article Turk J Agric For 35 (2011) 421-431 © TÜBİTAK doi: Isolation and characterization of native Bacillus thuringiensis isolates from Syrian soil and testing of their insecticidal activities against some insect pests Hassan AMMOUNEH*, Muhanad HARBA, Emad IDRIS, Hayat MAKEE Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, . BOX 6091 - SYRIA Received: Abstract: Bacillus thuringiensis was detected in of soil samples collected from different regions in Syria and 25 B. thuringiensis isolates were found to be highly toxic to larvae of Ephestia kuehniella Zeller, Phthorimaea operculella Zeller, and Cydia pomonella L. (Lepidoptera), but not to the larvae of Culex quinquefasciatus (Diptera). Light microscopy investigation showed the presence of bipyramidal and cuboidal parasporal bodies produced by these isolates. The existence of different cry genes in the tested isolates was studied using a PCR strategy with a set of general primers recognizing some of the cry genes reported in the relevant literature. Primers corresponding to 2 types of cry genes (cry1 and cry2) successfully amplified DNA in all of the tested isolates. Moreover, the proteins encoded by these genes were detected in the SDS-PAGE of the purified parasporal bodies. The 50% lethal concentration of the spore-crystal mixture of the 25 isolates against E. kuehniella larvae varied from to μg g-1. A comparison of the LC50 values of the tested isolates with those of the reference strains B. thuringiensis kurstaki HD-1 and HD-73 ( and 46 μg g-1, respectively) showed that some of these isolates have a higher toxicity potential. Moreover, flagellar serotyping revealed that 4 isolates, which were among the most toxic, belonged to serotype kurstaki. This study constitutes the first isolation and characterization of local B. thuringiensis isolates in Syria. Some .

• Nông nghiệp
• Bacillus thuringiensis
• Ephestia kuehniella
• Bacillus thuringiensis
• Microbial pesticides
• Rod shaped
• Luận văn Thạc sỹ Sinh học
• Nghiên cứu Bacillus Thuringiensis
• Đặc điểm sinh học Bacillus Thuringiensis
• Một số chủng Bacillus Thuringiensis
• Chủng Bacillus Thuringiensis
• Chủng sinh Protein tinh
• Diệt côn trùng cánh vảy
• Fungal extract
• Antibacterial activity
• Flacherie disease in mulberry silkworm
• Bacillus thuringiensis serotypes
• Cry protein
• Molecular mass
• Agro climate soils
• Bacillus thuringiensis isolated
• Bacillus thuringiensis strain Bt407
• Partial characterization
• Keratinase produced
• Bacillus thuringiensis strain Bt407 isolated
• Poultry soil
• Sàng lọc tổng hợp chitinase
• Khả năng sinh tổng hợp chitinase
• Chủng Bacillus thuringiensis phân lập ở Việt Nam
• Chế phẩm sinh học
• Phase contrast microscopy
• Btk HD 1 Bacillus thuringiensis var kurstaki HD 1
• Malachite green
• Vidarbha region
• Azadirachta indica
• Spodoptera litura
• Econeem plus
• Bacillus thuringiensis formulation
• Helicoverpa armigera
• Evaluation of native isolates
• Bacillus thuringiensis against
• Cotton bollworm
• Cry gene
• H armigera
• Native entomopathogenic bacillus thuringiensis
• Article cry gene profile
• Gene specific primers
• Solid state fermentation
• Hot water spring
• Yeast extract
• Substrate in solid state fermentation
• Bacillus thuringiensis J2
• Yeast extracts
• Bacillus thuringiensis var
• Kurstaki HD 1
• Production of biopesticide
• Nghiên cứu ung thư
• Vi khuẩn Gram dương hiếu khí
• Đa dạng chủng Bacillus thuringiensis
• Tinh thể protein độc
• Luận án tiến sĩ
• Luận án tiến sĩ Sinh học
• Vi sinh vật học
• Vi khuẩn Bacillus thuringiensis
• Sugarcane juice
• Biopolymer polyhydroxybutyrate
• Bacillus spp
• Nitrogen deficient
• Bacillus thuringiensis Berliner
• Cry1 primer
• Plodia interpunctella
• PCR positive B
• thuringiensis
• Thuốc trừ sâu vi sinh
• Rừng ngập mặn
• Hoạt tính diệt sâu
• Chủng vi khuẩn Bacillus
• Are Bt crop safe
• tài liệu Bt
• tìm hiểu Bt
• tài liệu Bacillus thuringiensis
• thuốc trừ sâu hoá học
• ô nhiễm môi trường
• thuốc trừ sâu sinh học
• chủng Bacillus thuringiensis diệt sâu tơ
• khả năng diệt sâu xanh trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo nông nghiệp
• Công nghệ hóa
• Ấu trùng muỗi
• Chế phẩm Bacillus thuringiensis subsp
• Israelensis
• Hoạt tính sinh học
• Hóa dược
• Hoạt tính diệt muỗi
• khả năng diệt sâu tơ
• nghiên cứu khoa học
• báo cáo khoa học
• vai trò nông nghiệp
• công nghiệp sinh học
• Characterization analysis
• Gene cloning
• Escherichia coli
• Polymerase chain reaction
• Bacillus thuringiensis Mm2
• Cry3Aa11 δ endotoxin
• Homologous gene expression
• Cry3Aa11 gene
• PCR amplified
• Bacillus thuringiensis IAM 12077
• Poly β hydroxybutyrate
• Random mutagenesis
• Nucleotide sequence
• Physiological properties