TAILIEUCHUNG - PCR detection of Fusarium oxysporum f. sp. radicis-lycopersici and races of F. oxysporum f. sp. lycopersici of tomato in protected tomato-growing areas of the eastern Mediterranean region of Turkey

Fusarium crown and root rot (caused by Fusarium oxysporum f. sp. radicis-lycopersici, FORL) and Fusarium wilt (caused by Fusarium oxysporum f. sp. lycopersici, FOL) are the most important diseases to affect tomatoes in protected growing conditions in the eastern Mediterranean region of Turkey. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2013) 37: 457-467 © TÜBİTAK doi: PCR detection of Fusarium oxysporum f. sp. radicis-lycopersici and races of F. oxysporum f. sp. lycopersici of tomato in protected tomato-growing areas of the eastern Mediterranean region of Turkey 1, 2 Ayşegül ÇOLAK *, Mehmet BİÇİCİ Biological Control Research Station, Adana, Turkey 2 Department of Plant Protection, Faculty of Agriculture, Çukurova University, Adana, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: Fusarium crown and root rot (caused by Fusarium oxysporum f. sp. radicis-lycopersici, FORL) and Fusarium wilt (caused by Fusarium oxysporum f. sp. lycopersici, FOL) are the most important diseases to affect tomatoes in protected growing conditions in the eastern Mediterranean region of Turkey. These diseases cause significant yield losses in this region. Polymerase chain reaction (PCR) studies were used to characterize 87 isolates from Adana and Mersin provinces, representative of different locations. Among them, 60% and 40% of the plants were determined to have FORL and FOL, respectively. FORL and FOL race 3 are the dominant pathogens in this region. Some differences between morphological identification and molecular detection (PCR) were observed. Key words: Fusarium oxysporum f. sp. lycopersici, Fusarium oxysporum f. sp. radicis-lycopersici, PCR detection, tomato 1. Introduction In covered tomato-growing areas in the eastern Mediterranean region, it was previously known that the wilt disease caused by Fusarium oxysporum f. sp. lycopersici (Sacc.) . Snyder & . Hans (FOL) was of prime importance. Later, crown and root-rot diseases caused by F. oxysporum f. sp. radicis-lycopersici Jarvis & Shoemaker (FORL) were encountered more frequently (Yücel and Çınar 1989; Can et al. 2004). F. oxysporum has spread .

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• Fusarium oxysporum f
• sp
• lycopersici
• Fusarium oxysporum f
• sp
• radicis lycopersici
• PCR detection
• Tomato growing
• Molecular detection
• In vitro
• Bacillus spp
• Fusarium oxysporum f sp ciceri
• Fusarium wilt of chickpea
• Fusarium wilt
• Antifungal potential
• Fusarium oxysporum
• Curcuma zedoaria
• Fusarium oxysporum F Sp Udum
• Curcuma zedoaria (christm )
• Antifungal efficiency of curcuma zedoaria
• Fusarium oxysporum f sp lycopersici
• Meloidogyne incognita
• Interaction between meloidogyne incognita
• Plant growth parameters
• Sporulation and temperature
• In vitro condition
• pH on growth
• sporulation of Fusarium oxysporum
• Fusarium oxysporum f sp lycopersici
• Selection and characterization
• Virulent Fusarium oxysporum
• Nutritional security
• Management of the disease becomes difficult
• In vitro evaluation
• In vitro evaluation of different fungicides
• Bioagents against Fusarium oxysporum
• Investigations were carried ou
• BMC Genomics
• Apium graveolens
• Coriandrum sativum
• Differentially expressed genes
• Fusarium oxysporum species complex
• Fusarium yellows
• Fusarium oxysporum f sp cucumerinum
• Cucumber Fusarium wilt
• Virulence variation
• Differentially expressed genes
• Novel specific primers
• Fusarium oxysporum f sp cubense
• Sybr green real time PCR
• Real time PCR
• The Fusarium wilt of banana
• Organic amendments
• Biocontrol of Fusarium wilt
• Tomato caused by Fusarium oxysporum
• Dual culture technique
• Petri dishes
• Pisum sativum
• Pathogenicity factors
• F oxysporum formae speciales
• Disease management
• Trichoderma spp
• ciceri
• Rhizoctonia solani
• Pathogen infested plots
• Foc–biocontrol interaction
• cubense
• Musa defense genes
• Suppression subtractive hybridization
• Banana plants
• Trichoderma asperellum
• Botanical extract
• In vivo (Greenhouse)
• Chitosan and plant extracts alone
• Weather parameters
• Correlation and PDI
• Effect of weather parameters on wilt
• Wilt (Fusarium oxysporum f sp gladioli)
• High economic return
• Chickpea wilt
• Characterization of Indian isolates
• Micro conidia
• Size of chlamydospores
• Panama wilt
• Banana in Bihar
• An extensive work
• Roving survey
• Panama wilt disease
• Fusarium oxysporum f sp cubense
• Seed borne
• Seed transmission
• Seeds of chickpea
• Pseudomonas fluorescens
• Trichoderma viride
• Bio control agents against
• Fungi toxicants
• Pigeonpea wilt
• In vivo
• Integrated disease management
• Azoxystrobin and soil drenching
• Morphological and molecular characteristics
• RAPD method
• Antagonistic action
• Trichoderma Isolates
• Fusarium oxysporum f sp lycopersci
• Studied microscopically
• Morphological characterization
• Colletrotrichum capsici
• Fusarium oxysporum f sp capsici
• Wilt disease in chilli
• Potential trichoderma strains
• Fusarium oxysporum f sp ricini
• Per cent inhibition
• In vitro efficacy
• Bio control agents against castor