TAILIEUCHUNG - Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation
This is the first report of successful regeneration of haploid lines in iris (Iris pseudacorus) developed by in situ parthenogenesis followed by embryo rescue. Native genotypes of I. pseudacorus (2n = 38) were prepared as female parents and I. spuria was set as the males. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2015) 39: 596-600 © TÜBİTAK doi: Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation 1,2, 3 4 Mohammad SADAT HOSSEINI GROUH *, Naeimeh SOUSARAEI , Mohammad AKBARI , 5 3 Vahid RAHIMI , Hamid Reza BAYAT 1 Department of Horticulture, Faculty of Agriculture, University of Jiroft, Jiroft, Iran 2 Department of Horticultural Crops, Faculty of Agriculture, University of Jiroft, Jiroft, Iran 3 Department of Horticulture, College of Abouraihan, University of Tehran, Tehran, Iran 4 Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran 5 Department of Biotechnology and Molecular Genetics, Faculty of Agriculture, University of Mohagheghe Ardabili, Ardabil, Iran Received: Accepted: Published Online: Printed: Abstract: This is the first report of successful regeneration of haploid lines in iris (Iris pseudacorus) developed by in situ parthenogenesis followed by embryo rescue. Native genotypes of I. pseudacorus (2n = 38) were prepared as female parents and I. spuria was set as the males. Anthers collected from the pollen donor were irradiated by X-ray with doses of 0, 100, 200, 300, and 400 Gy in 2012. A second round of experiments was conducted in 2013 with 300 and 400 Gy. The ovaries from each pollinated flower were collected and cultured and the produced plantlets were then acclimatized. Chromosome counting and flow cytometry were used to determine the ploidy levels. The viability of irradiated pollens was also evaluated. Pollination with irradiated pollen at doses ranging from 100 to 400 Gy can significantly reduce fruit set, particularly at higher doses (300 and 400 Gy). The embryo production was reduced in I. pseudacorus as doses increased; the highest doses led to reduction in the number of regenerants with the .
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