TAILIEUCHUNG - The global transcriptional response of a raw starch-degrading amylolytic Saccharomyces cerevisiae strain to oxygen limitation and genetic modification

This study investigates the global transcriptional response of Saccharomyces cerevisiae strain WTPB-G, which was developed by transforming laboratory strain FY23 with the pPB-G plasmid expressing the Bacillus subtilis α-amylase and the Aspergillus awamori glucoamylase as a fusion protein. | Turkish Journal of Biology Turk J Biol (2016) 40: 684-693 © TÜBİTAK doi: Research Article The global transcriptional response of a raw starch-degrading amylolytic Saccharomyces cerevisiae strain to oxygen limitation and genetic modification 1, 1 2 Ceyda KASAVI *, Serpil ERASLAN , Ebru TOKSOY ÖNER , Betul KIRDAR 1 Department of Chemical Engineering, Boğaziçi University, İstanbul, Turkey 2 Department of Bioengineering, Marmara University, İstanbul, Turkey Received: Accepted/Published Online: 1 Final Version: Abstract: This study investigates the global transcriptional response of Saccharomyces cerevisiae strain WTPB-G, which was developed by transforming laboratory strain FY23 with the pPB-G plasmid expressing the Bacillus subtilis α-amylase and the Aspergillus awamori glucoamylase as a fusion protein. Genome-wide analysis of transcript levels revealed the cellular mechanisms and the related pathways that were affected by the genetic modification, which conferred the ability of starch utilization to wild-type cells by the introduction of a plasmid-harboring gene encoding the amylolytic activity. Fermentations were carried out in media containing glucose as the only carbon source under aerated and microaerated conditions, and the effect of aeration was also investigated in terms of fermentation properties and transcriptional response. The genome-wide gene expression analysis highlighted that plasmid replication induced cell wall organization and biogenesis, and repressed approximately 20% of the genes involved in ribosome biogenesis and RNA processing. Although oxygen limitation was found to be less effective in the transcriptional changes, a link between oxygen limitation and genes involved in oxidation-reduction, pH reduction, phosphate-containing compounds, and lipid and fatty acid processes was observed. Key words: Amylolytic yeast, Saccharomyces cerevisiae, .

• Sinh học
• Amylolytic yeast
• Saccharomyces cerevisiae
• α amylase
• Phosphate containing compounds
• RNA processing
• Ethanol producing yeast
• Acid hydrolysis
• Oryza sativa
• Bioethanol production
• Amylolytic enzymes
• Chromatin
• Chromatin Collide
• Diverse Model Systems
• Genome Biology
• RNA binding protein
• Crosslinking and immunoprecipitation
• In vivo regulatory networks
• Molecular biology
• Lecture Molecular biology
• Sinh học phân tử
• Ribosomal RNA processing
• Transfer RNA processing
• RNA structure
• Regulating mRNA processing
• Polyadenylation sites
• Whole genome scale
• RNA editing
• RNA seq
• Genetic variation
• Post transcriptionally modified
• BMC Genomics
• Somatic cell reprogramming
• Alternative splicing
• Transcriptional networks
• Gene expression
• Gene regulation
• Pseudomonas aeruginosa
• Post transcriptional modification catalyzed
• ADAR enzymes
• Coding sequences
• Principles of biochemistry
• Lecture Principles of biochemistry
• Nguyên lý hóa sinh
• Biochemical compounds
• Transcriptional regulation
• Bài giảng Sinh học phân tử
• Cryptic transcripts
• Metabolic labeling
• RNA sequencing
• Transcripts undergo processing
• BMC Bioinformatics
• RNA binding residue
• Gradient tree boosting
• Structural neighborhood features
• Post transcriptional RNA processing
• scientific reports
• Report medicine
• traditional medicine
• medical knowledge
• medical research
• analysis of cytoplasmic
• BMC Plant Biology
• Trimethylguanosine synthase
• Asexual reproduction via seeds
• Normalization methods
• Colorectal cancer
• Current normalization methods
• Quality control
• Facilitate downstream analysis
• Subsequent analysis
• NGS data processing
• Data evaluation
• Bioinformatics workflow
• Stem cells
• Next generation sequencing
• Differential expression
• Differential transcript regulation
• Differential splicing
• Plant Biology
• Defective kernel
• Pentatricopeptide repeat proteins
• E subgroup PPR proteins
• Single cell
• Interactive graphical visualization
• Bioinformatic software
• Biological research
• Mammary gland
• Seasonal weight loss
• Signal transduction
• Single cell RNA seq
• Isoform estimate
• Exon inclusion ratios
• Whole exome sequencing
• Multiple processing steps
• health
• care
• primary
• report
• RNA
• Paula Grabowski
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học