TAILIEUCHUNG - Flavonoid dynamic responses to different drought conditions: amount, type, and localization of flavonols in roots and shoots of Arabidopsis thaliana L.

Flavonoids accumulate in plants in response to water deficit. Changes in amount, type, and localization of flavonoids under different drought conditions in Arabidopsis thaliana have not been well investigated. | Turkish Journal of Biology Research Article Turk J Biol (2016) 40: 612-622 © TÜBİTAK doi: Flavonoid dynamic responses to different drought conditions: amount, type, and localization of flavonols in roots and shoots of Arabidopsis thaliana L. 1,2 1, 2,3 Behrokh SHOJAIE , Akbar MOSTAJERAN *, Mustafa GHANNADIAN Plant Science Division, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran 2 Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran 3 Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran 1 Received: Accepted/Published Online: Final Version: Abstract: Flavonoids accumulate in plants in response to water deficit. Changes in amount, type, and localization of flavonoids under different drought conditions in Arabidopsis thaliana have not been well investigated. Therefore, in this study flavonoid patterns were investigated under water potentials of –, –, and – MPa at 0, 24, 48, 120, and 192 h after drought induction. Determination of amount and type of flavonoids was performed by HPLC and spectroscopy. In addition, localization of flavonoids was detected by DPBA staining and a fluorescent microscope. Only quercetin and kaempferol were detected in hydrolyzed extracts of roots and shoots. The maximum amounts of the above-mentioned flavonols were detected under severe drought stress. Under all drought conditions, there was more kaempferol than quercetin. Moreover, amounts of both flavonols and total flavonoids were greater in roots than in shoots. Different fluorescence intensities of the flavonoid-DPBA complex were observed in all seedlings from shoots to root tips. The results of this study suggest that flavonoid responses of Arabidopsis to drought stress are dynamic, and intensity and duration of drought .

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