TAILIEUCHUNG - Induction and development of anther-derived gametic embryos in cucumber (Cucumis sativus L.) by optimizing the macronutrient and agar concentrations in culture medium

The androgenic potentials of various cucumbers were significantly (P < ) influenced by the different macronutrient strengths and agar concentrations, although anther culture responses could vary according to the cucumber type. | Turkish Journal of Biology Turk J Biol (2016) 40: 571-579 © TÜBİTAK doi: Research Article Induction and development of anther-derived gametic embryos in cucumber (Cucumis sativus L.) by optimizing the macronutrient and agar concentrations in culture medium 1, 1 1 2 1 Mohammad Reza ABDOLLAHI *, Samereh NAJAFI , Hasan SARIKHANI , Sayyed Saeed MOOSAVI Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran 2 Department of Horticultural sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran Received: Accepted/Published Online: Final Version: Abstract: Anther culture response of a cucumber cultivar (Beta Alpha) and three Iranian landraces of cucumber (Basmenj, Isfahani, and Korki) on MS culture media with an altered macronutrient strength (half, full, , and double-strength) or various concentrations of agar (0, , 7, and 14 g L–1) was evaluated in two separate experiments. The effect of different concentrations of agar on the development of callus-derived embryos was also assessed. The androgenic potentials of various cucumbers were significantly (P < ) influenced by the different macronutrient strengths and agar concentrations, although anther culture responses could vary according to the cucumber type. A doubled strength of macronutrients in culture medium resulted in the highest percentage of callogenesis (100%) in Isfahani cucumber, while use of full strength of macronutrients produced the highest percentage of embryogenic calli () in the mentioned landrace compared to other tested forms. The maximum number of gametic embryos ( and embryos/anther) differentiated from Isfahani anther cultures on full- and half-strength MS medium, respectively. In the second experiment, the highest percentages of callogenesis were obtained in MS culture media solidified with 14 g L–1 and 7

• Sinh học
• Cucumis sativus L
• Anther culture
• Agar concentration
• Embryo maturation
• Cotyledonary embryos
• Cucumber (Cucumis sativus L
• Genetic advance
• Genetic variability in cucumber
• Variance revealed significant
• Evaluation of cucumber
• Cucumber (Cucumis sativus L )
• Cucumber (Cucumis sativus L ) genotypes
• Hill zone of Karnataka
• Sirsi Local 2 exhibited superiority
• The amount of irrigation water for cucumber
• Cucumis sativus L grown on four types of substrate
• The amount of irrigated water
• The highest absolute yield
• Fungal pathogens associated
• Spoilage of cucumber
• Cucumber (Cucumis sativus L ) fruits
• Fungal isolates
• Agromorphological traits
• Cucumis sativus
• Pollen grains
• Palynological studies in Cucumber
• Gene effects
• Generation mean analysis
• Gene effects for fruit yield
• Calcium humate
• Boron humate
• Boron uptake of tomato
• Tomato (Lycopersicon esculentum L
• Relative water content
• Superoxide dismutase
• Ascorbate peroxidase
• Cucumber Cucumis sativus L
• Total chlorophyll content
• Zucchini yellow mosaic virus
• Molecular markers
• Cucurbit crops including cucumber
• Amplified fragment length polymorphism
• Sequence related amplified polymorphism
• Gene action
• Parents and hybrids
• Quality traits in cucumber
• Gene action for yield
• Genetic diversity
• Tocher’s method
• Intra and inter cluster distance
• Yield parameters in cucumber
• PSB and Trichoderma viride
• Organic nutrient management
• Growth and flowering
• Investigation entitled
• Traits and phenotypic selection etc
• Factor causing genetic diversity
• Related and geographical
• Yield traits
• NAA and pusa uday
• Yield and yield traits
• Yield traits of cucumber
• Foliar application
• Plant growth regulators
• Testing of hybrid vigour
• Sex phenology in cucumber
• Hybrid vigour for sex phenology
• Desirable significant positive heterosis
• Poly house
• Growth and yield of cucumber
• Irrigation levels
• Yield of cucumber
• Xác định lượng nước tưới phù hợp
• Cây dưa leo
• Lượng nước tưới cho cây dưa leo
• Bốn loại giá thể
• Growth regulators
• Polyhouse condition
• Plant growth regulators on growth
• Quality parameters in cucumber
• Protein profiling
• SDS PAGE
• Monoecious cucumber (Cucumis sativus L )
• Seed protein profiles
• Genetic variability
• Additive gene
• Protected structure
• Genetic advance in cucumber
• Downy mildew
• Downy mildew in cucumber
• Fungicide mixtures against Pseudoperonospora cubensis
• Quantity of cucumber in countries
• Indirect selection
• Seed traits
• Fruit yield in cucumber
• Seed traits with fruit yield in cucumber
• Genetic divergence
• Horticultural traits in cucumber
• Divergence studies
• Exotic sources were planted
• Quantitative traits
• Cucumber (Cucumis sativus L ) germplams
• Genetic variability among 78 cucumber
• Cucumber germplasm collected