TAILIEUCHUNG - Molecular markers to evaluate genetic diversity among Venturia inaequalis isolates obtained from apple plantations in Isparta Province

Turkey, the third leading apple producer in world, produces approximately million tons of apples per year. Venturia inaequalis, the causal agent of apple scab, is a major fungal disease among apples varieties. This study was conducted in accordance with the plant protection method for isolates collected from the apple production areas in Isparta Province. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2016) 40: 489-498 © TÜBİTAK doi: Research Article Molecular markers to evaluate genetic diversity among Venturia inaequalis isolates obtained from apple plantations in Isparta Province 1 2, 3 Suat KAYMAK , Nuh BOYRAZ *, Jon DANIELS Plant Protection Central Research Institute, Ankara, Turkey 2 Department of Plant Protection, Faculty of Agriculture, Selçuk University, Konya, Turkey 3 Department of Entomology & Plant Pathology, Oklahoma State University, Stillwater, OK, USA 1 Received: Accepted/Published Online: Final Version: Abstract: Turkey, the third leading apple producer in world, produces approximately million tons of apples per year. Venturia inaequalis, the causal agent of apple scab, is a major fungal disease among apples varieties. This study was conducted in accordance with the plant protection method for isolates collected from the apple production areas in Isparta Province. The purpose of this study was to determine genetic differences among V. inequalis isolates causing apple scab, and to discriminate the effects of varieties and geographical origins on genetic diversity. For this purpose, a total of 83 samples from the leaves and fruits of infected plants were collected to obtain isolates from apple scab. Of these, 67 isolates were obtained by isolating single conidia. Genetic differences and the relationship of the isolates were evaluated using random amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), simple sequence repeat (SSR), and sequence-related amplified polymorphism (SRAP) markers. The marker techniques SRAP and ISSR were used for the first time in this study to determine the genotype of the isolates of V. inaequalis. There was no relationship between apple varieties and geographic isolation or source of cultivar patterns. Variance analysis and molecular

• Nông nghiệp
• Apple scab
• Genetic diversity
• Venturia inaequalis
• Apple plantations in Isparta Province
• Major fungal disease among apples varieties
• Severity of apple scab
• Uttarakhand Hills
• Scab incidence
• Apple scab in Uttarakhand Hills
• Etiology and disease cycle
• Disease cycle of apple scab
• Apple scab in Harsil
• Gangotri fruit belt
• Biology and management
• Apple scab incited
• Quality of apple
• Effectively managing apple scab
• Integrated spay schedules against
• Powdery mildew
• Marsonina blotch
• Apple scab disease
• Spray schedules for controlling apple scab
• Marker based screening of F1
• F1 (Firdous x Gala) mapping population
• Major scab resistance gene Rvi6
• Gene Rvi6 in apple
• Apple (Malus × Domestica)
• Apple cultivars
• Conventional treatment
• Field application
• Organic farming
• Populin treatment
• Venturia inaequalis (Cooke ) G Wint
• Cow urine
• Uria apple
• Spring ascospore production
• Pre leaf fall spray of chemical