TAILIEUCHUNG - Optimization of monoclonal antibody expression in CHO cells by employing epigenetic gene regulation tools

Monoclonal antibodies (mAbs) are crucial in pharmaceutical biotechnology. Mammalian cell lines are the most preferred for their production. One of the significant challenging issues of mammalian expression systems is epigenetic gene silencing. | Turkish Journal of Biology Turk J Biol (2017) 41: 622-628 © TÜBİTAK doi: Research Article Optimization of monoclonal antibody expression in CHO cells by employing epigenetic gene regulation tools 1 1 1 1 Fatemeh NEMATPOUR , Fereidoun MAHBOUDI , Vahid KHALAJ , Behrouz VAZIRI , 1 2 1 1, Samira AHMADI , Maryam AHMADI , Saedeh EBADAT , Fatemeh DAVAMI * 1 Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran 2 Department of Medical Biotechnology, Semnan University of Medical Sciences, Semnan, Iran Received: Accepted/Published Online: Final Version: Abstract: Monoclonal antibodies (mAbs) are crucial in pharmaceutical biotechnology. Mammalian cell lines are the most preferred for their production. One of the significant challenging issues of mammalian expression systems is epigenetic gene silencing. Employing epigenetic gene regulation tools can increase the productivity of the mammalian cell lines. Sodium butyrate (NaBut) and valproic acid (VPA) regulate gene expression by inhibiting histone deacetylase. A ubiquitous chromatin-opening element (UCOE) can improve expression by reducing DNA methylation. Here, the separate and combined effects of NaBut and VPA histone deacetylase inhibitors (iHDACs) and UCOE on mAb synthesis were studied. Stable cell lines were generated by non-UCOE (CHO-HL) and UCOE-containing vectors (CHO-UHUL) and cultured in the presence and absence of NaBut or VPA. Expression analysis showed that CHO-UHUL gave a 4-fold greater yield than non-UCOE CHO-HL. Antibody production levels of the CHO-HL and CHO-UHUL cells increased 2-fold and after NaBut and VPA treatment, respectively. These results indicate that UCOE has more impact on antibody expression than iHDACs. iHDAC treatment exhibited at least a higher antibody yield in UCOE containing CHO-UHUL cells. Thus utilization of NaBut and VPA (iHDACs) and UCOE resulted in .

• Sinh học
• Monoclonal antibodies
• Chinese hamster ovary
• Epigenetic gene silencing
• Chromatin opening element
• Sodium butyrate
• Valproic acid
• Chromatography A
• Ion exchange
• Downstream processing
• Foot and mouth disease virus
• Whole virion particle
• 12S subunits
• BMC Biotechnology
• Humanized rats
• Human antibodies
• Human idiotypes
• Recombinant antibodies
• Mammalian cell based screening mammalian cell culture
• Protein production
• Ammonium sulfate
• Multiplex analysis
• Therapeutic monoclonal antibody
• Hybridomas
• Interleukin 6
• Supplemental media
• Kháng thể đơn dòng
• Sản xuất kháng thể đơn dòng
• Monoclonal antibodies Mab
• Kháng thể đa dòng
• 8 bước tạo MAb
• Tinh chế MAb
• Monoclonal antibodies specific
• Serum progesterone
• Hybrid cells
• Hybridoma technology
• Enzyme linked immunosorbent assay
• Virus like particles
• Porcine circovirus 2
• PCV2 associated diseases
• BMC Cancer
• Meta analysis
• Multiple myeloma
• Randomised controlled trials
• Rabbit anti mouse serum albumin polyclonal antibody generation
• Release testing of monoclonal antibodies
• Murine ascites fluid
• Mouse serum albumin
• Polyclonal antibody
• Protein A affinity chromatography
• Host cell proteins
• Downstream bioprocessing
• Antibody genes
• B cell receptors
• Comparative efficacy
• Different methods
• Generation of 12S particles
• 146S particles of FMDV serotype
• Specific monoclonal antibodies
• Dengue virus
• NS1 protein
• Mouse immunization
• HEK cells
• Monoclonal antibody
• Serum free medium
• Gel filtration chromotography
• Tobacco mosaic virus
• Hybrid cell
• Steroid hormones
• Serum albumine
• C terminal amidation
• Genetic modification
• Recombinant monoclonal antibody
• Peptidylglycine α amidating monooxygenase
• BMC Developmental Biology
• Monoclonal antibody screen
• Immunological resources
• Molecular mechanisms
• Schmidtea mediterranea
• Breast cancer
• Ovarian cancer
• Ovarian cancer patients
• Quantify serum CA125 concentration
• Superficially porous particles
• Low hydrophobicity
• Reversed phase liquid chromatography
• Ion exchange chromatography
• Mab biosimilars
• Cation exchange chromatography
• Continuous chromatography
• Process development
• Protein A
• Integrated continuous downstream process
• Mild purification
• Process design