TAILIEUCHUNG - Analysis of CA125 antigen in normal human seminal plasma highlights the molecular heterogeneity of underlying glycosylated species

Cancer antigen CA125 represents an extracellular part of transmembrane mucin MUC16 and may be expressed in tissues of the male reproductive tract. It reaches human seminal plasma (hSP) after undergoing the main processes involved in protein speciation: Synthesis, posttranslational modifications, compartmentalization, and auto/proteolytic degradation. | Turkish Journal of Biology Turk J Biol (2017) 41: 543-551 © TÜBİTAK doi: Research Article Analysis of CA125 antigen in normal human seminal plasma highlights the molecular heterogeneity of underlying glycosylated species Ninoslav MITIC*, Bojana MILUTINOVIC, Miroslava JANKOVIC Department for Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy-INEP, University of Belgrade, Belgrade, Serbia Received: Accepted/Published Online: Final Version: Abstract: Cancer antigen CA125 represents an extracellular part of transmembrane mucin MUC16 and may be expressed in tissues of the male reproductive tract. It reaches human seminal plasma (hSP) after undergoing the main processes involved in protein speciation: synthesis, posttranslational modifications, compartmentalization, and auto/proteolytic degradation. This study was aimed at profiling CA125-immunoreactive species in hSP from healthy subjects as a specific and unexplored source of this tumor-associated antigen expressed under normal physiological conditions. High molecular mass components from total hSP and corresponding acid-soluble hSP preparations were analyzed. The results indicated that antibodies recognizing two distinct immunogenic areas of CA125 antigen exhibited a common pattern of immunoreactive bands affected by low pH and reducing agents. They comprise a large, heavily glycosylated moiety and a mixture of low glycosylated species ranging from 100 to 150 kDa. High molecular mass CA125immunoreactive smears overlapped core 2 O-glycans and the Lex glycotope, the latter also being abundant on distinct lower molecular mass immunoreactive bands. Within the bulk of normal hSP mucins, the CA125 antigen is present in low amounts and resides on heterogeneously glycosylated species that may be proteolysis-derived and sensitive to redox environments. Both factors influence the composition of hSP .

• Sinh học
• Human seminal plasma
• Conformational epitope
• Proteolytic degradation
• Transmembrane mucin MUC16
• Pathological conditions
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• kiến thức y học
• nghiên cứu y học
• Investigation on the occurrence
• Pathological conditions affecting broilers
• Mortality patterns associated
• Maximum followed
• báo cáo khoa học
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• cách trình bày báo cáo
• BMC Veterinary Research
• Salivary levels
• Disease detection
• Trace elements
• Rectal prolapse
• Visual evoked potentials
• Test retest
• Electrical potentials