TAILIEUCHUNG - The role of predicted spermidine family transporters in stress response and cell cycle in Schizosaccharomyces pombe

Fission yeast Schizosaccharomyces pombe has a variety of stress-signaling proteins that protect cells against environmental or intracellular stress. These proteins help the cells to respond to stress conditions and regulate intracellular functions such as cell division or gene expression. | Turkish Journal of Biology Research Article Turk J Biol (2017) 41: 419-427 © TÜBİTAK doi: The role of predicted spermidine family transporters in stress response and cell cycle in Schizosaccharomyces pombe Aslıhan ÖRS GEVREKCİ* Department of Psychology, Faculty of Science and Letters, Başkent University, Ankara, Turkey Received: Accepted/Published Online: Final Version: Abstract: Fission yeast Schizosaccharomyces pombe has a variety of stress-signaling proteins that protect cells against environmental or intracellular stress. These proteins help the cells to respond to stress conditions and regulate intracellular functions such as cell division or gene expression. Polyamines (spermidine, spermine, and putrescine) are known to be important in the regulation of stress response and cell division. In this study, we tried to experimentally characterize novel S. pombe genes that are involved in the polyamine pathway and understand their potential roles. Sequence analysis revealed four genes that code for (predicted) spermidine family transporters in S. pombe. In an attempt to characterize these (predicted) spermidine family transmembrane transporters and their possible roles, deletion mutants of these candidate genes were created. These mutants were exposed to different stress conditions, such as DNAdamaging agents and osmotic stress, to understand their significance in the stress response. Next, the mutants were analyzed in terms of cell size, growth rate, and spore formation to understand their contribution to cell cycle control. The results revealed that individual deletion of two of these genes, and , resulted in sensitivity to DNA-damaging agents, indicating their role in DNA damage response. Key words: Polyamines, spermidine, Schizosaccharomyces pombe, cell cycle, stress response 1. Introduction Polyamines (spermidine, spermine, and putrescine) .

• Sinh học
• Polyamines spermidine
• Cell cycle
• Schizosaccharomyces pombe
• Stress response
• Gene expression
• BMC Plant Biology
• Salinity–alkalinity stress
• Chlorophyll precursor
• Antioxidant system
• Polyamines protect plants against
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• Cell lines used
• BMC Bioinformatics
• Single cell RNA seq
• Differential gene expression
• Stochastic gene expression
• Transcriptional bursting model
• Tomato expression database
• NGS data analysis
• Expression Atlas
• Gene expression comparison
• Gene expression data
• Gene network
• Simulate gene
• Stochastic simulation algorithm
• Escherichia coli M15
• Burkholderia cepacia in escherichia coli
• Catechol 1
• 2 dioxygenase gene
• Improved expression of catechol 1
• Genus Citrus
• Gene function
• Co expression networks
• Genome wide scale
• Brassica spp
• Digital gene expression
• Brassica includes
• Diploid progenitors
• Displaying complex
• The inflammatory gene expression
• Horse oil in DNCB treated mice skin
• The inflammatory gene expression by horse oil
• DNCB treated mice skin
• Including severe erythema
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• Brinjal leaves through agroinfiltration
• Transient expression of reporter gene