TAILIEUCHUNG - Effects of plant growth regulators and sucrose on the regeneration of Paphiopedilum micranthum var. North Vietnam

The objective of this study was to determine the effects of 2,4- Dichlorophenoxyacetic acid (2,4-D) combined with 6- Benzylaminopurine (BAP) and sucrose on the shoot and root regeneration of Paph. micranthum var. North Vietnam. Young fresh leaves were cut into small explants and then cultured on media containing 2,4-D (, , or ppm). | Vietnam Journal of Agricultural Sciences ISSN 2588-1299 VJAS 2018; 1(1): 11-20 Effects of Plant Growth Regulators and Sucrose on the Regeneration of Paphiopedilum micranthum var. North Vietnam Vu Tien Binh1,2 and Sorgan S. K. Tai2 1 Faculty of Agronomy, Vietnam National University of Agriculture, Hanoi 131000, Vietnam; 2 Department of Biotechnology, National Formosa University, Yunlin County 632, Taiwan Abstract Received: September 27, 2017 Accepted: January 25, 2018 The objective of this study was to determine the effects of 2,4Dichlorophenoxyacetic acid (2,4-D) combined with 6Benzylaminopurine (BAP) and sucrose on the shoot and root regeneration of Paph. micranthum var. North Vietnam. Young fresh leaves were cut into small explants and then cultured on media containing 2,4-D (, , or ppm). Within each of the 2,4-D concentration, BAP was added at three different concentrations (, , or ppm) for callus induction after 4 weeks. Shoot tips (2-3 mm in length) from the leaf-derived calluses were then transferred vertically on half-strength MS medium (2,4-D combined with BAP (, , or ppm) for shooting, and rootless shoots with 2 leaves were transferred to the same MS medium for rooting after 4 months of culture. Rootless shoots with 2 leaves were also transferred vertically on half-strength MS medium with different sucrose concentrations (0, 10, 20, 30, or 40 g L-1) to optimize the growth and development of the plantlets. The results indicated that the optimum degree of shoot and root regeneration occurred on halfstrength MS medium containing ppm 2,4-D combined with ppm BAP. Approximately of the cultures responded with shoots and leaves/explant. In addition, under the optimal concentrations, the number and length of roots per explant after 4 months of culture were roots per explant and an average root length of cm. MS medium supplemented with 30 g L-1 sucrose had .

• Sinh học
• Vietnam journal of agricultural sciences
• Plant growth regulators
• Sucrose on the regeneration
• Paphiopedilum micranthum var
• North Vietnam
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