TAILIEUCHUNG - High quality DNA isolation method for chickpea genotypes

In chickpea breeding genetic studies of individual plants need to be evaluated at the DNA level using molecular markers. A simple and reliable DNA extraction method is a prerequisite. This small-scale method is cetyltrimethylammonium bromide (CTAB)-based and extracts DNA from 1 to 3 folded young leaves processed in a ml tube with ml of extraction buffer and homogenized using an electric drill. | Turk J Biol 29 (2005) 1-5 © TÜB‹TAK High Quality DNA Isolation Method for Chickpea Genotypes Hasibe C‹NG‹LL‹, Abdülkadir AKÇ‹N Gebze Institute of Technology, Department of Biology, 41400, Çay›rova, Gebze, Kocaeli - TURKEY Received: Abstract: In chickpea breeding genetic studies of individual plants need to be evaluated at the DNA level using molecular markers. A simple and reliable DNA extraction method is a prerequisite. This small-scale method is cetyltrimethylammonium bromide (CTAB)based and extracts DNA from 1 to 3 folded young leaves processed in a ml tube with ml of extraction buffer and homogenized using an electric drill. Compared with the micro-prep method the improved mini-prep CTAB method is highly efficient and much cheaper in terms of time, chemical use and labor input. About 49 samples per day can easily be processed by one person. The DNA yield is greater (60 µg per 50-100 µg of fresh leaf tissue) than that obtained from the micro-prep method (50 mg from 5 g of fresh leaf tissue). High quality DNA was obtained and used successfully for restriction endonuclease digestion and polymerase chain reaction amplifications using the mini-prep CTAB method. Key Words: Chickpea, DNA isolation, Molecular markers Abbreviations: CTAB, Cetyltrimethylammonium bromide; ISSR, Inter simple sequence repeat; PCR, Polymerase chain reaction; RAPD, Random amplified polymorphic DNA; SSR, Simple sequence repeat. Nohut Genotiplerinde Yüksek Kalitede Saf DNA ‹zolasyon Metodu Özet: Moleküler mark›rlardan faydalan›larak ekonomik aç›dan önemli birçok bitkinin ›slah edilmesi, günümüzde giderek önemi artan mark›r teknolojisinin ve mark›r yard›m›yla seleksiyon uygulamalar›n›n, art›k rutin çal›flmalar olabilece¤i konusunu gündeme getirmifltir. Bu nedenle, öncelikli olarak çal›flmalarda kullan›lacak ana materyal olan DNA’n›n, k›sa sürede ve saf olarak eldesi son derece önemlidir. Bunun için de güvenilir bir DNA izolasyon metodunun belirlenmesi gerekmektedir. Bu .

• Sinh học
• Chickpea genotypes
• DNA isolation
• Molecular markers
• Polymerase chain reaction
• Cetyltrimethylammonium bromide
• Genetic variability
• G X E interaction
• Stability parameter
• Diverse environments
• Yield stability in chickpea genotypes
• Physiological and salinity
• Physiological responses
• Varying salinity levels
• Chickpea (Cicer arietinum)
• Genetics variability
• Genetic character variability
• Desi chickpea
• Desi chickpea (Cicer arietinum L ) genotypes
• Nodules per plant
• Helicoverpa armigera
• Genotypes accepted
• Screening of different genotypes
• Chickpea against gram pod borer
• Gram pod borer
• Genetic diversity
• Inter Intra cluster distance
• Genetic diversity in reference set
• Callosobruchus chinensis
• Callosobruchus chinensis L
• Laboratory conditions
• Pulse beetle
• Certain chickpea genotypes against
• Inoculation and resistance
• Biochemical parameters
• Ascochyta blight infected moderately resistant
• Susceptible chickpea genotypes
• Inoculated leaves
• Phenotypic coefficient of variance
• Genotypic coefficient of variance
• Heritability and genetic advance
• Per cent of mean
• Chickpea genotypes (Cicer arietinum L )
• Wilt complex
• Inoculum level
• Complex pathogens
• Reaction of ruling chickpea genotypes
• Quantification of minimum inoculum level
• Cicer arietinum
• Characterization of chickpea
• Genotypes through RAPD analysis
• Largest grown food legume
• Sclerotium rolfsii
• Trichoderma harzianum
• Collar rot of chickpea
• Management of collar rot
• Chickpea (Cicer aeritinum L )
• Total nitrogen content in chickpea
• Possible involvement
• Exogenous gibberellic acid
• Nitrogen assimilating enzyme activity
• Fuarium wilt
• Sick plot and green house
• Green house
• Sick plot
• Artificial condition
• Screening of chickpea germplasm
• Fusarium wilt
• Cultivars and resistance
• Screening of chickpea varieties
• Chickpea varieties
• Chickpea Cicer arietinum L
• Water potential
• Osmotic adjustment
• Water use efficiency
• Drought tolerant genotypes
• Seed yield per plant
• Indirect selection
• Association and path coefficient analysis
• White kabuli (MNK 1)
• Genotype screening
• Temperate agro climatic conditions
• Resistance against wilt
• Plant height
• Shoot weight and root weight
• Rhizoctonia Bataticola
• Genotypes against Pratylenchus Thornei
• Cicer arietinum L
• Genetic variability of Chickpea
• Plant population
• Net returns
• Seed rate
• Genotypes in response to seed rate
• Economics of kabuli chickpea
• Cultivated chickpea Cicer arietinum L
• Ascochyta blight
• Ascochyta rabiei
• Seed yield
• Yield criteria
• genotypes
• Path coefficient analysis
• Profile and genetic
• Evaluation of elite chickpea
• Genetic diversity analysis
• Characterization and evaluation
• RAPD primers
• Random amplified polymorphic DNA
• Heat tolerance chickpea
• Molecular characterization
• Osmotic potential
• Total soluble carbohydrates
• Chickpea (Cicer arietinum L )