TAILIEUCHUNG - Biodiesel Feedstock Production from Freshwater Microalgae Grown in Sugarcane Juice Hydrolysate

Microalgae have the potential to generate significant quantities of biomass and lipid suitable for biodiesel preparation. Heterotrophic growth of microalgae involves the utilization of organic compounds as sole carbon and energy sources under dark condition that could get rid of the dependence on light. | Journal of Clean Energy Technologies, Vol. 4, No. 4, July 2016 Biodiesel Feedstock Production from Freshwater Microalgae Grown in Sugarcane Juice Hydrolysate Supaporn Kookkhunthod, Rattanaporn Baojungharn, and Ratanaporn Leesing disadvantages including low biomass productivity, low lipid content and long periods of cultivation. Hence, heterotrophic and mixotrophic cultures have been proposed as feasible alternatives for the production of biomass and cellular lipid accumulation [5]. Mixotrophic cultures of microalgae have an edge over photoautotrophic cultures as they have two energy sources as organic carbon source and light, they can simultaneously drive photoautotrophic and heterotrophic to utilize both inorganic (CO2) and organic carbon substrates [6], [7]. While, heterotrophic growth of microalgae involves the utilization of organic compounds as sole carbon and energy sources under dark condition that could get rid of the dependence on light [8]. Based on different microalgae and culture conditions such as temperature, nutrient and light intensity, lipid content and fatty acid composition are varied, the cellular lipid content in various classes of microalgae was improved significantly under stress conditions, such as nitrogen starvation and phosphate limitation [9], [10]. The microalgae Chlorella sp., especially C. protothecoides and C. vulgaris are two widely available microalgae strains in the commercial applications [11]. Chlorella sp. KKU-S2 is a freshwater microalgae found in the area of Khon Kaen, northeastern of Thailand [12], can grow under photoautotrophic, heterotrophic and mixotrophic conditions and the most fatty acids produced by these strain are palmitic acid, stearic acid, oleic acid and linoleic acid which a suitable feedstock for biodiesel production. Nowadays, the production cost of microalgae-derived biodiesel is much higher than that of diesel derived from petroleum fossil fuel due to the lower culture process efficiency and higher cost

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